青海野生与栽培麻花艽psbA-trnH序列的比较 |
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引用本文: | 张得钧,高庆波,李福安,李永平,俞科贤.青海野生与栽培麻花艽psbA-trnH序列的比较[J].安徽农业科学,2011,39(10):5780-5781. |
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作者姓名: | 张得钧 高庆波 李福安 李永平 俞科贤 |
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作者单位: | 青海大学医学院,青海西宁,810001;中国科学院西北高原生物研究所,青海西宁,810001 |
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基金项目: | 青海大学中青年科研基金项目(2009-QY-19) |
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摘 要: | 目的]分析野生与栽培麻花艽(Gentiana straminea Maxim.)psbA-trnH序列的差异,为麻花艽的基源鉴定和品质评价提供分子依据。方法]采用PCR扩增纯化后直接测序的方法,测定野生与栽培麻花艽cpDNA psbA-trnH核苷酸序列并作序列同源性分析。结果]野生与栽培麻花艽的cpDNA psbA-trnH片段长度分别为316和317 bp,两者之间有4个碱基的变异。结论]利用psbA-trnH区序列的差异可以鉴别野生与栽培麻花艽。
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关 键 词: | 麻 花艽(Gentiana straminea Maxim.) psbA-trnH 区序列 聚合醇链反应 同源性分析 |
Comparison of Chloroplast DNA psbA-trnH Sequences between Wild and Cultivated Gentiana straminea Maxim.in Qinghai |
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Institution: | ZHANG De-jun et al(Medical College,Qinghai University,Xining,Qinghai 810001) |
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Abstract: | Objective]To distinguish Gentiana straminea Maxim.from wild and cultivated plants by comparing chloroplast DNA psbA-trnH sequences,so as to provide a molecular basis for orgin identification and quality evaluation.Method] cpDNA psbA-trnH sequences of Gentiana straminea Maxim.were amplified by polymerase chain reaction(PCR),and then sequenced by direct PCR sequencing method for homologous analysis.Result] The lengths of cpDNA psbA-trnH of wild and cultivated plants were 316 and 317 bp respectively,and there were 4 variable sites.Conclusion] The nucleotide differences of psbA-trnH regions could be used for distinguishing Gentiana straminea Maxim.from wild and cultivated plants. |
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Keywords: | Gentiana straminea Maxim psbA-trnH sequences PCR Homologous analysis |
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