| 多糖水解酶活力的测定方法研究进展 |
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| 引用本文： | 张永勤,王哲平,孙岩,詹志春.多糖水解酶活力的测定方法研究进展[J].安徽农业科学,2009,37(32):15643-15645. |
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| 作者姓名： | 张永勤 王哲平 孙岩 詹志春 |
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| 作者单位： | 青岛科技大学化工学院,山东青岛,266042;湖北省武汉新华扬生物有限公司,湖北武汉,430074 |
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| 摘 要： | 综述了目前常用的几种多糖水解酶活力的测定方法，并介绍了一些最新涌现的方法。还原糖测定法由于具有简便、快速、低成本、可高通量、实时测定的特点，仍是当前酶活力测定中的首推方法，其中灵敏度较高、可较准确地测定酶解初速度的3-甲基-2．苯并噻唑酮腙（MBTH）法将更加受到业界的推崇。灵敏度普遍较高的荧光法如果能克服底物成本较高，制备过程中分子量降低等问题，也将得以广泛应用。
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| 关 键 词： | 多糖水解酶 还原糖端基 底物显色 荧光法 3-甲基-2-苯并噻唑酮腙(MBTH) |
Research Progress of Determination Method of Polysaccharide Hydrolase Activity |
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| ZHANG Yong-qin et al.Research Progress of Determination Method of Polysaccharide Hydrolase Activity[J].Journal of Anhui Agricultural Sciences,2009,37(32):15643-15645. |
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| Authors: | ZHANG Yong-qin |
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| Institution: | ZHANG Yong-qin et al (College of Chemical Engineering,Qingdao University of Science & Technology,Qingdao,Sh,ong 266042) |
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| Abstract: | Several determination methods of polysaccharide hydrolase activity were reviewed,and some novel methods were introduced.With the simple,rapid,low cost,high-throughput and real-time determination characteristics,the reducing sugar ends assay was still the most important method for the determination of polysaccharide hydrolase activity,in which the 3-methyl-2-benzothiazolinone hydrozone (MBTH) method will be more precise owing to its relatively high sensitivity and accuracy in the initial velocity measurement... |
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| Keywords: | Polysaccharides hydrolases Reducing sugar ends Chromophore-labled substrate Fluorescence 3-methyl-2-benzothiazolinone hydrozone (MBTH) |
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