利用转基因牛生产重组人乳铁蛋白研究 |
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引用本文: | 杨鹏华,倪凤娥,李宁.利用转基因牛生产重组人乳铁蛋白研究[J].安徽农业科学,2008,36(35). |
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作者姓名: | 杨鹏华 倪凤娥 李宁 |
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作者单位: | 1. 长江大学生命科学学院,湖北荆州,434025;中国农业大学农业生物技术国家重点实验室,北京,100094 2. 长江大学生命科学学院,湖北荆州,434025 3. 中国农业大学农业生物技术国家重点实验室,北京,100094 |
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基金项目: | 国家高技术研究发展计划(863计划)生物反应器重大专项基金 |
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摘 要: | 目的]研究利用转基因牛生产重组人乳铁蛋白,为利用转基因动物生产药用或食用蛋白提供依据。方法]对已获得的人乳铁蛋白转基因牛进行人工催乳,用乳成分分析仪分析了转基因对乳汁的影响,并利用放免法检测了重组人乳铁蛋白在转基因牛乳中的表达。结果]重组人乳铁蛋白在牛乳中获得了高效表达,表达水平为(3.429±0.417)mg/ml。通过转基因牛乳的乳成分分析发现,转基因牛乳与常乳在乳脂、总蛋白、乳糖和干物质的含量上没有明显变化。结论]重组人乳铁蛋白在转基因牛乳中获得了高效表达,为大规模生产重组人乳铁蛋白奠定了基础。
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关 键 词: | 转基因 重组人乳铁蛋白 牛 |
Study on the Production of Recombinant Human Lactoferrin in Transgenic Cattle |
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Abstract: | Objective] The research aimed to study the production of recombinant human lactoferrin in transgenic cattle,provid the basis for production of pharmaceutical or edible proteins in transgenic animals.Method] Transgenic cattle expressing recombinant human lactoferrin was induced to lactation.The effects of transgene to milk components were analyzed with a milk components analyzer.The expression level was determined by radioimmunoassay.Result] The recombinant human lactoferrin was expressed in transgenic cattle at high level of(3.429±0.417) mg/ml.The investigations of milk components showed that the basic composition,such as fat,total protein,lactose and dry matter,of transgenic milk was similar to that of non-transgenic milk.Conclusion] The recombinant human lactoferrin was expressed effectively in milk of cloned transgenic cattle,which provided the basis for the large-scale production of the recombinant human lactoferrin. |
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Keywords: | Transgene Recombinant human lactoferrin Cattle |
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