异柠檬酸裂解酶抑制剂对L-谷氨酸合成的影响

目的]研究琥珀酸和苹果酸对L-谷氨酸合成的影响。方法]以谷氨酸棒杆菌的典型茵株Corynebacterium glutamicum ATCC 13032为供试菌株，研究琥珀酸和苹果酸对细胞生长、L-谷氨酸的合成、发酵液中的残糖量以及细胞中异柠檬酸裂解酶活性的影响。结果]琥珀酸对异柠檬酸裂解酶的活性具有部分抑制作用。在0-5．0g／L范围内，琥珀酸的添加提高了L-谷氨酸发酵的糖酸转化率，细胞生长和L-谷氨酸分泌受到抑制，残糖量增大。苹果酸的添加降低了异柠檬酸裂解酶的活性和L-谷氨酸的产量，残糖量和细胞生长没有明显的规律性变化。同时添加琥珀酸和苹果酸各2．0g/L时，异柠檬酸裂解酶的活性和细胞生长都有所下降，L-谷氨酸的产量和残糖量没有明显变化。结论]添加琥珀酸增加了L-谷氨酸的产量，添加苹果酸则降低了L-谷氨酸的产量。

Influences of Isocitrate Lyase Inhibitors on the Synthesis of L-Glutamate

Objective] The purpose was to research the influences of succinic acid and malic acid on the synthesis of L-glutamate.Method] With the typical strain Corynebacterium glutamicum ATCC 13032 of Corynebacterium glutamate as tested strain,the influences of succinic acid and malic acid on cell growth,synthesis of L-glutamate,residual sugar content in fermentation broth and activity of isocitrate lyase in cell were researched.Result] The succinic acid had partial inhibition on the activity of isocitrate lyase.I...