激素因子对铁皮石斛离体培养开花诱导的效应

目的]筛选铁皮石斛离体培养开花诱导的适宜激素因子。方法]采用铁皮石斛茎段离体培养的试管苗为试验材料,以MS为基本培养基,进行单因子激素处理（PP333、TDZ的不同浓度梯度）和多因子激素处理（PP333、TDZ、6-BA以及NAA的不同组合）的比较试验,研究激素因子对铁皮石斛离体培养开花诱导的效应。结果]单因子激素处理中,PP333、TDZ的适宜浓度和其诱导的花芽形成率分别为0.2 mg/L和8.5%、0.06 mg/L和15.5%;多因子激素处理对开花诱导的效应依次为：PP333＋6-BA＋NAA＋TDZ组合处理〉PP333＋6-BA＋NAA组合处理〉PP333＋6-BA组合处理和PP333＋NAA组合处理;适宜的激素组合为PP3330.3 mg/L＋6-BA 0.5 mg/L＋NAA 0.5mg/L＋TDZ 0.06 mg/L,其花芽形成率和花开放率高达80.4%和90.3%。结论]PP333和TDZ对铁皮石斛离体培养开花诱导具有重要影响,TDZ的效应大于PP333。适宜的TDZ浓度与其他激素的合理配比,有利于花芽的形成。

Effects of Hormone Factors on Flowering Induction in vitro Culture of Dendrobium Officinate Kimura et Migo

Objective] The aim was to select the suitable hormone factors for flowering induction in vitro culture of Dendrobium officinate Kimura et Migo.Method] The test-tube plantlets from the stems of Dendrobium officinate Kimura et Migo were used as the experimental materials and MS medium as the basic medium,comparative tests were carried out between single-factor hormone treatments(different concentrations of PP333 and TDZ) and multi-factor hormone treatments(different combinations of PP333,TDZ,6-BA and NAA) t...