白腐真菌降解硝基苯化合物的研究

目的]研究白腐真菌的生长及降解硝基苯化合物的情况。方法]使用紫外分光光度计测量不同培养条件下锰过氧化物酶酶活，确定白腐真菌最佳培养条件。用气相色谱检测，通过比较降解前后的峰面积值算出硝基苯降解率。结果]白腐真菌的最佳培养条件为：温度37℃，醋酸缓冲液调pH值为4．5，吐温80作表面活性剂。白腐真菌的最佳培养时间为6d。白腐真菌使浓度10mg／L硝基苯完全降解的时间为7d。结论]白腐真菌为易培养、高效降解茵，能实现硝基苯的无害化降解目标。

Studies on the Nitrobenzene Biodegradation of White Rot Fungi

Objective] The research aimed to study on the nitrobenzene biodegradation of white rot fungi.Method] UV was used to detect the activity of manganese peroxidase to obtain the optimal culture environment and GC to confirm the rate of nitrobenzene degradation.Result] The optimal culture environment of the white rot fungi was as follows: 37 ℃,pH value of 4.5,tween 80 as surfactant.The optimal culture time of white rot fungi are six days and 10 mg/L nitrobenzene can be degraded entirely in seven days.Conclusion] The white rot fungi is a kind of culturable,efficient microbe that can degrade nitrobenzene to harmless substance entirely.