首页 | 本学科首页   官方微博 | 高级检索  
     检索      

玉米EMS突变体库构建及突变体初步鉴定
引用本文:樊双虎,郭文柱,路小铎等.玉米EMS突变体库构建及突变体初步鉴定[J].安徽农业科学,2014(11):3162-3165,3185.
作者姓名:樊双虎  郭文柱  路小铎等
作者单位:西南科技大学生命科学与工程学院;华中农业大学植物科学技术学院;中国农业科学院生物技术研究所;齐鲁师范学院生命科学学院;
基金项目:山东省高校科研发展计划项目(J13LJ02)
摘    要:目的]构建玉米诱变变体库,创制玉米新种质资源.方法]采用甲基磺酸乙酯(EMS)诱变玉米优良自交系B73和郑58,构建变体库;选取小叶夹角突变体rla1、rla2进行密植产量测定和遗传学分析.结果]2个突变体库的M2代含有表型丰富的突变体,尤其是一些突变体表现出优良农艺性状,如叶夹角小、棕色叶中脉、矮化、抗性,具有重要的育种价值.rla1、rla2表型相似,较初始材料耐密植,在试验各种植密度下,单位面积产量均高于对照组;且产量随着密度增大而提高,其最大密植度在100000株/hm2.遗传学分析显示,rla1、rla2都是单基因隐性突变,而且是等位基因突变.结论]B73和郑58的EMS突变体库为玉米育种提供了宝贵的材料资源.

关 键 词:甲基磺酸乙酯  突变体库  种质资源  叶夹角

Construction and Preliminary Identification of EMS Mutant Library in Maize
FAN Shuang-hu,GUO Wen-zhu,LU Xiao-duo,ZHANG Chun-yi.Construction and Preliminary Identification of EMS Mutant Library in Maize[J].Journal of Anhui Agricultural Sciences,2014(11):3162-3165,3185.
Authors:FAN Shuang-hu  GUO Wen-zhu  LU Xiao-duo  ZHANG Chun-yi
Institution:1.School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, Sichuan 621010; 2.Biotechnology Institute of CAAS, Beijing 100081; 3.School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, Sichuan 621010 ; Biotechnology Institute of CAAS, Beijing 100081;)
Abstract:Objective] The aim was to construct mutant libraries of maize and create new maize germplasm resources.Method] Mutant libraries of maize inbred B73 and Zheng 58 were constructed by EMS mutagenesis.Measurement the yield under high density and genetic analysis of rla1 and rla2 was carried out.Result] The M2 generation contained various phenotypes of mutants.Above all,some mutants showed excellent agronomic traits,such as small leaf angle,brown leaf midrib,dwarfism and resistance,which were very valuable in maize breeding.The narrow leaf angle mutants rla1 and rla2 had similar phenotype,and allowed higher planting densities than the wild type line.Under different planting densities in this study,the yields of rla1 and rla2 per unit area were higher than control,and increased as the increase of planting density,whose proper maximum density would be more than 100 000 plants per hectare.The results of genetics analysis indicated that the mutant phenotype of rla1 and rla2 was caused by recessive mutation in a single locus,and rla1 and rla2 were two alleles of the same gene.Conclusion] The EMS mutant libraries of B73 and Zheng 58 provided valuable germplasm resources for maize breeding.
Keywords:Ethyl methane sulfonate  Mutant library  Germplasm resource  Leaf angle
本文献已被 CNKI 维普 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号