| 白亮独活的组织培养和植株再生研究 |
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| 引用本文： | 谭兴,杨军,陈德灿,龚真才.白亮独活的组织培养和植株再生研究[J].安徽农业科学,2006,34(20):5182-5183,5222. |
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| 作者姓名： | 谭兴 杨军 陈德灿 龚真才 |
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| 作者单位： | 1. 西华师范大学生命科学学院,四川,南充,637002
2. 西华师范大学生命科学学院,四川,南充,637002;西华师范大学珍稀动植物研究所,四川,南充,637002 |
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| 基金项目： | 四川省重点学科建设项目（SZD0420）资助. |
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| 摘 要： | 建立了白亮独活组织培养和小植株再生的体系。以叶片为外植体,在含不同生长调节物质的MS培养基中培养,结果表明,在MS培养基上附加2,4-D 2.0 mg/L和6-BA 0.5 mg/L对愈伤组织的诱导率可达100%;而愈伤组织继代最合适的培养基培养为附加2,4-D2.0 mg/L和6-BA0.2 mg/L的MS培养基;苗的诱导以附加6-BA2.0 mg/L的MS培养基效果最好;生根在附加NAA0.2mg/L和IAA0.5 mg/L的1/2 MS培养基中最好。
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| 关 键 词： | 白亮独活 叶片 组织培养 植株再生 |
| 文章编号： | 0517-6611（2006）20-5182-02 |
| 收稿时间： | 2006-06-15 |
| 修稿时间： | 2006-06-15 |
Research on the Tissue Culture and Plantlet Regeneration of Heracleum candicans |
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| TAN Xing, et al.Research on the Tissue Culture and Plantlet Regeneration of Heracleum candicans[J].Journal of Anhui Agricultural Sciences,2006,34(20):5182-5183,5222. |
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| Authors: | TAN Xing |
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| Institution: | School of Life Sciences, China West Normal University, Nanchong, Sichuan 637002 |
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| Abstract: | A system of tissue culture and plantlet regeneration of Heracleum candicans was established.The different combinations of plant growth regulators were added into MS medium to investigate their effects on tissue culture of leaf as explants and 100% callus was inducted on MS media with 2.0 mg/L 2,4-D and 0.5 mg/L 6-BA.And the most suitable subculture medium was MS with 2,4-D 2.0 mg/L and 6-BA 0.2 mg/L.Shoots were proliferated on MS media supplemented with 6-BA 2.0 mg/L and the rooting medium was 1/2 MS media added with NAA 0.2 mg/L and IAA 0.5 mg/L. |
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| Keywords: | Heracleum candicans Leaf Tissue culture Plantlet regeneration |
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