| 花粉管通道法转化影响因素的分析 |
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| 引用本文： | 刘明,杨君,安利佳.花粉管通道法转化影响因素的分析[J].安徽农业科学,2007,35(12):3483-3483,3523. |
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| 作者姓名： | 刘明 杨君 安利佳 |
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| 作者单位： | 大连理工大学环境生命学院生物科学与生物工程系,辽宁大连,116024;大连理工大学环境生命学院生物科学与生物工程系,辽宁大连,116024;大连理工大学环境生命学院生物科学与生物工程系,辽宁大连,116024 |
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| 基金项目： | 国家科技部项目(JY04-B-01) |
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| 摘 要： | 花粉管通道法转化过程中外源基因转化途径、转化受体细胞、转化时间是重要的影响因素;结合大豆花粉管通道形成与受精过程,突出受体细胞的感受态的获得,对转化操作要点加以分析,为花粉管通道法的进一步研究指明方向.
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| 关 键 词： | 花粉管通道法 转化途径 转化受体细胞 转化时间 受体细胞感受态 |
| 文章编号： | 0517-6611（2007）12-03483-01 |
| 收稿时间： | 2007-01-27 |
| 修稿时间： | 2007-01-27 |
Analysis of the Factor Impacting the Gene Transferring with the Pollen-tube Pathway Method |
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| LIU Ming ,et al.Analysis of the Factor Impacting the Gene Transferring with the Pollen-tube Pathway Method[J].Journal of Anhui Agricultural Sciences,2007,35(12):3483-3483,3523. |
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| Authors: | LIU Ming |
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| Institution: | Department of Bioseience and Biotechnology, Dalian University of Technology, Dalian, Liaoning 116024 |
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| Abstract: | It is important to improve the transformation frequency of the pollen-tube pathway method,and we analyzed its impact factor,including the pathway that foreign gene was transferred into embryo and the recipient cell with the pollen-tube pathway method.Soybean being taken as an example,two physiological processes were jointed together: the growth of pollen-tube pathway and double-fertilization,to emphasize the importance of the appropriate time and its competent state for the transformation manipulation of flowering plant,and the direction of the future research was pointed out. |
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| Keywords: | Pollen-tube pathway method Gene transfer pathway Gene transfer time Recipient cell Recipient cell competent state |
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