藻蓝蛋白α亚基裂合酶的分子克隆和酶动力学研究 |
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引用本文: | 苏平,周明.藻蓝蛋白α亚基裂合酶的分子克隆和酶动力学研究[J].安徽农业科学,2012,40(1):36-38,40. |
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作者姓名: | 苏平 周明 |
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作者单位: | 湖北师范学院生命科学学院,湖北黄石,435002;华中农业大学农业微生物学国家重点实验室,湖北武汉,430070 |
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基金项目: | 湖北省教育厅科学技术研究资助项目,湖北师范学院人才引进项目,高效博士学科专项科研基金批准项目 |
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摘 要: | 为了比较研究不同藻种中藻蓝蛋白裂合酶CpcE/F的结构与功能的差异,对Anabaena sp.PCC 7120中的CpcE/F进行克隆,并进行大量表达,将表达的裂合酶CpcE/F用于藻蓝胆素(PCB)与Mastigocladus laminosus PCC 7603藻蓝蛋白α-亚基(α-PC)脱辅基蛋白(CpcA)的体外重组,得到天然活性的α-PC,从而表明CpcE/F所编码的蛋白质是α-PC生物合成的裂合酶,并对CpcE/F的酶动力学进行了初步研究。
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关 键 词: | 鱼腥藻 藻蓝蛋白 CpcE CpcF 重组 酶动力学 |
Study on Molecular Cloning and Enzyme Kinetics of Anabaena α-Phycocyanin Lyase |
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Institution: | SU Ping et al(College of Life Sciences,Hubei Normal University,Huangshi,Hubei 435002) |
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Abstract: | To comparatively study the differences on structures and functions of phycocyanin lyase CpcE/F from different cyanobacteria,CpcE/F from Anabaena sp.PCC 7120 were cloned and expressed greatly.The reconstitution of PCB and CpcA from Mastigocladus laminosus PCC 7603 in vitro using the overexpressed lyase Anabaena sp.PCC 7120 CpcE/F showed that CpcE/F from Anabaena sp.PCC 7120 is the specific enzyme for biosynthesis of α-PC,furthermore,the enzyme kinetics of PcE/F lyase were studied preliminarily. |
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Keywords: | Anabaena Phycocyanin CpcE lyase CpcF lyase Reconstitution Enzyme kinetics |
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