坛紫菜丝状体类囊体膜和光系统Ⅱ的分离鉴定

目的]为深入研究坛紫菜的光合生理奠定基础。方法]以坛紫菜丝状体为材料，用蔗糖密度梯度超速离心法分离纯化类囊体膜和光系统Ⅱ（PsⅡ）颗粒。结果]色素带B1的吸收峰分别位于419、498、540、565和676nm，色素带B2、B3、B2．a、B2．b、B3．a．B3．b和B3．C的吸收峰分别位于419．436、485和676nm。类囊体膜B2的放氧速率和DCIP光还原活性分别为12μmol（O2）／mg（Chl）·h]和2．3aeq／mg（cm）·h]；B3的分别为37μmol（O2）／mg（chl）·h]和25ueq／mg（Chl·h）]；B3-b的分别为567Ixmol（O2）／mg（Cra）·h]和168ueq／nag（Chl）·h]；B3-c的分别为8．1μmol（O2）／nag（Chl）·h]和23ueq／mg（Chl）·h]；B2-b只有DcIP光还原活性（168ueq／mg（Cra）·h]）。结论]该研究为坛紫菜丝状体类囊体膜和光系统Ⅱ的分离鉴定提供了试验依据。

Isolation and Characterization of Thylakoid Membrane and Photosystem Ⅱ from Conchocelis of Porphyra haitanesis

Objective] The purpose was to lay a foundation for researching the photosynthetic physiology of Porphyra haitanesis thoroughly.Method] With the conchocelis of P.haitanesis as material,the thylakoid membranes and photosystem Ⅱ(PSⅡ) particles were isolated and purified by sucrose density gradient ultracentrifugation.Result] The absorption peaks of pigment band B1 were at 419,498,540,565 and 676 nm resp.and that of B2,B3,B2-a,B2-b,B3-a,B3-b and B3-c were at 419,436,485 and 676 nm resp.The oxygen release rate and DCIP photoreduction activity of thylakoid membrane B2 were 12 μmol(O2)/mg(Chl)·h]and 2.3 ueq/mg(Chl)·h] resp.,that of B3 were 37 μmol(O2)/mg(Chl)·h] and 25 ueq/mg(Chl)·h] resp.,that of B3-b were 567 μmol(O2)/mg(Chl)·h] and 168 ueq/mg(Chl)·h] resp.,that of B3-c were 8.1 μmol(O2)/mg(Chl)·h] and 23 ueq/mg(Chl)·h] resp.and B2-b only had DCIP photoreduction activity(168 ueq/mg(Chl)·h]).Conclusion] The research provided test basis for isolating and identifying the thylakoid membranes and PSⅡ from the conchocelis of P.haitanesis.