| 马MxA基因第12外显子多态性检测研究 |
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| 引用本文： | 菊林花,金花,呼都特.马MxA基因第12外显子多态性检测研究[J].安徽农业科学,2009,37(8):3463-3464. |
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| 作者姓名： | 菊林花 金花 呼都特 |
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| 作者单位： | 内蒙古农业大学动物科学与医学学院,内蒙古呼和浩特,010018;内蒙古锡林郭勒职业学院医学系,内蒙古锡林浩特,026000 |
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| 基金项目： | 内蒙古自治区自然基金资助项目(200508010413) |
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| 摘 要： | 目的]建立检测马MxA基因第12外显子多态性的快速、准确方法。方法]采用错配聚合酶链反应（mismatchPCR）对马MxA基因第12外显子进行扩增，对PCR产物进行限制性片段长度多态性分析（RFLP），鉴定MxA基因cDNA第1790位核苷酸点突变，并对PCR产物进行核苷酸序列分析。结果]马MxA基因第12外显子区域存在AA、AB、BB3个基因型；位于cDNA序列第l790位点的碱基发生变异（由汕c），引起了MxA蛋白编码区第562氨基酸由色氨酸变成半胱氨酸的变异；使用mismatchPCR—RFLP法所得PCR产物特异性序列，与RFLP分析结果相符。结论]采用mismatch PCR—RFLP对马MxA基因12外显子的多态性进行检测操作简单，结果准确。
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| 关 键 词： | 马 MxA基因 多态性 |
Study on Detection of Polymorphism of No.12 Exon in MxA Gene of Horse |
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| JU Lin-hua et al.Study on Detection of Polymorphism of No.12 Exon in MxA Gene of Horse[J].Journal of Anhui Agricultural Sciences,2009,37(8):3463-3464. |
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| Authors: | JU Lin-hua |
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| Institution: | JU Lin-hua et al(College of Animal Science , Veterinary Medicine of Inner Mongolia Agricultural University,Hohhot,Inner Mongolia 010018) |
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| Abstract: | Objective] The experiment aimed to establish a fast and accurate method to detect the polymorphism of No.12 exon in MxA gene of horse.Method] The mismatch PCR was used to amplify the No.12 exon,then the PCR product was conducted RFLP.It was concluded from the identification that the nucleotide 1 790 site in cDNA of MxA gene was mutated and the PCR product was undergone nucleotide sequence analysis.Result] There were 3 genotypes(AA,AB,BB) in the region of No.12 exon in MxA gene of horse.The bases of nucle... |
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| Keywords: | Horse MxA gene Polymorphism |
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