首页 | 本学科首页   官方微博 | 高级检索  
     检索      

栉江珧核糖体转录间隔区的初步研究
引用本文:严加坤,杨爱国,吴彪,周丽青.栉江珧核糖体转录间隔区的初步研究[J].安徽农业科学,2013,41(1):23-25,82.
作者姓名:严加坤  杨爱国  吴彪  周丽青
作者单位:中国水产科学研究院黄海水产研究所,山东青岛266071;上海海洋大学水产与生命学院,上海201306;中国水产科学研究院黄海水产研究所,山东青岛,266071
摘    要:目的]对栉江珧的核糖体转录间隔区(ITS)序列进行分析研究。方法]利用特定引物对栉江珧(Atrina pectinta)转录间隔区ITS-1和ITS-2的序列片段进行PCR扩增及测序,并分析片段的碱基序列特征。结果]栉江珧ITS-1序列长度为570 bp(含20.2%T、30.0%C、22.3%A、27.5%G)、ITS-2长度为618 bp(含22.3%T、27.3%C、23.5%A、26.9%G);与魁蚶、栉孔扇贝、牡蛎、菲律宾蛤仔进行比对,栉江珧ITS-1的相似性在25.6%~38.1%之间,ITS-2的则在24.5%~38.9%之间,说明栉江珧与试验所选的4种贝类之间的进化关系较远,种间变异很大,ITS序列可用于该纲物种的分类及鉴别。结论]该研究为我国栉江珧的种质资源保护和利用提供了一定的分子生物学依据和参考。

关 键 词:栉江珧  核糖体  转绿间隔区

Primary Study of Ribosomal DNA Internal Transcribed Spacers of Atrina pectinta
Institution:YAN Jia-kun et al(Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao,Shandong 266071)
Abstract: Objective ] The aim was to analyze the sequence of ribosomal DNA internal transcribed spacers (IriS) of Atrina pectinta. Meth- od] The ITS-1 and IT5-2 sequence tYagments of A. pectinta were amplitied and sequenced by specific primers, then their hase sequence fea tures was analyzed. Result] The ITS-I sequen(e length of A. pectinta was 570 bp ( including 20.2% T, 30.0%C, 22.3% A, 27.5% G), the ITS-2 was 618 bp (including 22.3%T, 27.3% C, 23.5% A, 26.9% G) ; the similarity of A. pectinta ITS-1 with Scapharca brotlghtonii, Chlamys.hrrei,Crassostrea virginica, Ruditapes phi/ippinarum was between 25.6% and 38.1% , while the ITS-2 was from 24.5% to 38.9% , it showed that the A. pectinta had highly divergent in genetic distance with the other fore' kinds of shelf and could be used for genetic diversity study at A. pectinta population lexel. Conclusion] It provided important molecular intbrmation for the proleetion and utilization of A. pectinla resourees of China.
Keywords:Atrina pectinta  Ribosome  Internal transcribed spacer
本文献已被 CNKI 维普 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号