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甜叶菊组培快繁技术研究
引用本文:胡松梅.甜叶菊组培快繁技术研究[J].安徽农业科学,2013,41(1):41-42.
作者姓名:胡松梅
作者单位:湖南娄底职业技术学院,湖南娄底,417000
基金项目:湖南省教育厅科研基金项目
摘    要:目的]研究甜叶菊的组培快繁技术。方法]以甜叶菊茎段为外植体,筛选其最佳外植体消毒条件、最佳不定芽增殖及壮苗生根培养基。结果]甜叶菊外植体材料的灭菌最佳条件为75%酒精20 s,0.1%升汞5~8 min;最佳不定芽增殖培养基为MS+1.0 mg/L6-BA+0.1 mg/L NAA,此时不定芽增殖率达80%;最佳壮苗生根培养基为1/2MS+0.1 mg/L 6-BA+0.1 mg/L NAA+0.2 mg/L IBA。结论]该研究为甜叶菊的工厂化规模生产提供了科学依据。

关 键 词:甜叶菊  快繁  不定芽  生根

Research on Tissue Culture and Rapid Propagation Technology of Stevia rebaudiana
HU Song-mei.Research on Tissue Culture and Rapid Propagation Technology of Stevia rebaudiana[J].Journal of Anhui Agricultural Sciences,2013,41(1):41-42.
Authors:HU Song-mei
Institution:HU Song-mei(Loudi Polytechnic,Loudi,Hunan 417000)
Abstract: Objective ] The aim was to study the tissue culture and rapid propagation technology of Stevia rebaudiana. Method ] The best ex plants sterilization method, optimal media of adventitious bud proliferation and seedling rooting were screened by using shoot tips of S. rebaudi- aria as explants. Result] The best explants sterilization method was to sterilized with 0.1% HgC12 for 5 - 8 min after being treated with 75% alcohol for 20 s ; tbe optimal adventitious bud proliferation medium was MS + 1.0 mg/L 6-BA + 0.1 mg/L NAA and the adventitious bud pro- liferation rate was 80% ; the optimal seedling rooting medium was 1/2MS + 0.1 mg/L 6-BA + 0.1 mg/L NAA + 0.2 mg/L IBA. Conclusion ] This study Drovided a scientific basis for the industrialized and larze-scale production of S. rebaudiana.
Keywords:Stevia rebaudiana  Rapid propagation  Adventitious bud  Rooting
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