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顶空固相微萃取-气质联用分析红富士苹果中的芳香物质
引用本文:赵峰,王少敏,高华君,薛培生,孙山.顶空固相微萃取-气质联用分析红富士苹果中的芳香物质[J].山东农业大学学报(自然科学版),2006,37(2):181-184,189.
作者姓名:赵峰  王少敏  高华君  薛培生  孙山
作者单位:山东省果树研究所,山东,泰安,271000
基金项目:教育部科学技术研究项目
摘    要:以红富士苹果为试材,采用顶空固相微萃取法提取芳香成分,用气相色谱—质谱联用仪进行定性、定量分析。结果表明,从红富士苹果中共检测鉴定出55种芳香成分,主要有酯类25种、烯烃类11种、醛类7种、醇类6种、酮类2种、羧酸类2种及其他化合物2种。其中酯类、醛类和醇类化合物的含量最高,分别为42.67%、29.85%和19.09%,可将红富士苹果归为“酯类型”品种。初步认定红富士苹果芳香成分的合成前体主要为脂肪酸(亚油酸和亚麻酸)和支链氨基酸。顶空固相微萃取—气质联用技术操作简单,能够很好地应用于苹果芳香成分的检测。

关 键 词:红富士苹果  顶空固相微萃取  气相色谱-质谱联用  芳香物质  芳香前体
文章编号:1000-2324(2006)02-0181-05
收稿时间:2005-12-10
修稿时间:2005-12-10

DETERMINATION OF AROMA COMPOUNDS OF ‘RED FUJI’ APPLE USING HEADSPACE SOLID-PHASE MICRO-EXTRACTION AND GC/MS
ZHAO Feng,WANG Shao-min,GAO Hua-jun,XUE Pei-sheng,SUN Shan.DETERMINATION OF AROMA COMPOUNDS OF ‘RED FUJI’ APPLE USING HEADSPACE SOLID-PHASE MICRO-EXTRACTION AND GC/MS[J].Journal of Shandong Agricultural University,2006,37(2):181-184,189.
Authors:ZHAO Feng  WANG Shao-min  GAO Hua-jun  XUE Pei-sheng  SUN Shan
Institution:Shandong Institute of Pomology, Taian 271000,China
Abstract:Determination on aroma compounds of 'Red Fuji' apple with headspaee solid-phase micro-extraction(HS-SPME) and GC/MS showed that 55 aroma compounds were detected and they were consist of 25 esters,11 alkenes, 7 aldehydes,6 alcohols,2 ketones,2 acids and 2 other compounds.Esters,aldehydes and alcohols are the most aboundant aromas,the content being 42.67%,29.85% and 19.09%,respectively,So "Red Fuji" apple was classified as ester type cultivar.It was considered that fatty acids(ic.linoleic acid and linolenic Acid) and branched amino acids are two main precursors for aroma compounds in 'Red Fuji' apple.HS-SPME and GC/MS is a convenient method for analyzing aroma compounds in apple.
Keywords:'Red Fuji' apple  Headspace solid-phase micro-extraction  GC-MS  Aroma compounds  precursor for aromas
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