| 小麦矮秆新基因的RAPD标记 |
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| 引用本文： | 于东海,李静,王秀芹,王洪刚.小麦矮秆新基因的RAPD标记[J].山东农业大学学报(自然科学版),2006,37(3):359-362. |
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| 作者姓名： | 于东海 李静 王秀芹 王洪刚 |
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| 作者单位： | 1. 山东农业大学农学院,山东泰安,271018
2. 枣庄市农科院,山东枣庄,277300 |
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| 摘 要： | 以矮秆易位系山农31504-1和高秆种质系山农298为材料,利用BSA法对矮秆基因进行了RAPD标记。从300个十碱基随机引物中筛选出引物S152可在矮秆DNA池中扩增出特征带。利用100株F_2单株进行连锁分析表明,引物S152扩增的RAPD标记与矮秆基因连锁,遗传距离为10．73±3．31cM。该标记可用于分子标记辅助选择,并为矮秆基因的深入研究奠定基础。
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| 关 键 词： | 小麦 矮秆基因 RAPD BSA |
| 文章编号： | 1000-2324（2006）03-0359-04 |
IDENTIFICATION OF RAPD MARKERS LINKED TO NEW WHEAT DWARF GENE |
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| YU Dong-hai,LI Jing,WANG Xiu-qin,WAGN Hong-gang.IDENTIFICATION OF RAPD MARKERS LINKED TO NEW WHEAT DWARF GENE[J].Journal of Shandong Agricultural University,2006,37(3):359-362. |
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| Authors: | YU Dong-hai LI Jing WANG Xiu-qin WAGN Hong-gang |
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| Abstract: | In this work,cross between dwarf translocation line shannong 31504-1 and high germplasm line shannong 298 was carried out for constructing F_2segrigation population,and bulked segregating analysis(BSA) was used to search for random amplified polymorphic DNA(RAPD)markers linked to the new dwarf gene.300 decamer primers were screened to identify one RAPD marker(S152_(2100))linked to the new dwarf gene.Using the F2 mapping population,the dwarf gene was shown to be closely linked to the marker S152_(2100)at a genetic dis- tance of 10.73 3.31cM.This marker can be conveniently used for maker-assisted selection in wheat breeding programs for the identification and pyramiding the dwarf gene with other genes. |
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| Keywords: | Wheat Dwarf gene RAPD BSA |
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