| BmNPV DNA解旋酶基因酵母双杂交诱饵载体的构建 |
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| 引用本文： | 杨金宏,王代钢,卢从德,孔卫青.BmNPV DNA解旋酶基因酵母双杂交诱饵载体的构建[J].山东农业大学学报(自然科学版),2010,41(2). |
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| 作者姓名： | 杨金宏 王代钢 卢从德 孔卫青 |
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| 作者单位： | 安康学院,陕西省蚕桑重点实验室,陕西,安康,725000 |
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| 基金项目： | 陕西科技厅农业攻关项目，安康学院基金 |
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| 摘 要： | 为了筛选家蚕对BmNPV DNA解旋酶的受体基因,根据GenBank公布的BmNPV (T3株)的序列,设计引物对其DNA解旋酶基因的核心结构域进行PCR扩增,成功克隆该基因并利用双酶切亚克隆至酵母双杂交诱饵载体PGBKT7.实验证明重组诱饵载体不能自激活,可以作为诱饵进行蛋白结合试验.
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| 关 键 词： | 家蚕核型多角体病毒 DNA解旋酶 诱饵载体 自激活 |
CONSTRUCTION OF YEAST TWO HYBRID SYSTEM BAIT VECTOR WITH DNA HELICASE OF BMNPV |
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| YANG Jin-hong,WANG Dai-gang,LU Cong-de,KONG Wei-qing.CONSTRUCTION OF YEAST TWO HYBRID SYSTEM BAIT VECTOR WITH DNA HELICASE OF BMNPV[J].Journal of Shandong Agricultural University,2010,41(2). |
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| Authors: | YANG Jin-hong WANG Dai-gang LU Cong-de KONG Wei-qing |
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| Institution: | YANG Jin-hong,WANG Dai-gang,LU Cong-de,KONG Wei-qing*(Ankang University,Key Sericultural Laboratory of Shaanxi,Ankang 725000,China) |
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| Abstract: | In object to screening the receptor gene of silkworm to BmNPV,basing on the genebank Nucleotides sequence of BmNPV(T3 strain),PCR was performed to amplify the sequence of DNA helicase core domain,and successfully cloned this gene,then sub-cloned this gene into yeast two hybrid bait vector pGBKT7.pGBKT7-hel was successfully recombined and had no self-activation,and can be used in yeast two hybrid as bait vector. |
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| Keywords: | BmNPV DNA helicase bait vector self-activation |
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