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| 核桃炭疽病高效生防菌株鉴定及抑菌活性 | |
| 引用本文： | 王清海,牛赡光,刘幸红,刘玉升,金莹.核桃炭疽病高效生防菌株鉴定及抑菌活性[J].山东农业大学学报(自然科学版),2011,42(3):335-337. |
| 作者姓名： | 王清海 牛赡光 刘幸红 刘玉升 金莹 |
| 作者单位： | 1. 山东省林业科学研究院,济南,250014 2. 山东农业大学植物保护学院,泰安,271018 3. 黄岛出入境检验检疫局,青岛,266000 |
| 摘 要： | 在对土壤有益微生物的分离、筛选中获得4株对核桃炭疽病菌具有较强抑制活性的拮抗细菌(编号分别为PF-1,PF-2,Bs-03,Bf-02)。通过对菌株的形态特征、染色反应、培养性状、生理生化特征、染色反应、培养性状、生理生化特性等试验项目,初步鉴定Bf-02为坚强芽孢杆菌(Bacillus firmus),Bs-03为枯草芽孢杆菌(Bacil-lus subtilis),PF-1为幼虫芽孢杆菌(Bacillus lacivae),PF-2为短芽孢杆菌(Bacillus brevis)。采用平板对峙培养法测定4株拮抗细菌对核桃炭疽病菌抑菌活性,4株拮抗细菌对核桃炭疽病菌具有较强的抑制活性,抑菌率均达到83%以上,抑制活性最强的是PF-2,抑菌率为88.47%,与其它3株拮抗细菌的抑菌率在P<0.05水平上具有显著性差异。其次为PF-1,抑菌率为86.11%,Bs-03和Bf-02的抑菌率在P<0.05水平上无显著性差异。 |
| 关 键 词： | 拮抗细菌 鉴定 芽孢杆菌 抑菌效果 |
IDENTIFICATION OF FOUR ANTAGONISTIC BACTERIA AND ITS ANTIFUNGAL ACTIVITY AGAINST COLLETOTRICHUM GLOEOSPORIOIDES |
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| WANG Qing-hai,NIU Shan-guang,LIU Xing-hong,LIU Yu-sheng,JIN Ying.IDENTIFICATION OF FOUR ANTAGONISTIC BACTERIA AND ITS ANTIFUNGAL ACTIVITY AGAINST COLLETOTRICHUM GLOEOSPORIOIDES[J].Journal of Shandong Agricultural University,2011,42(3):335-337. | |
| Authors: | WANG Qing-hai NIU Shan-guang LIU Xing-hong LIU Yu-sheng JIN Ying |
| Institution: | WANG Qing-hai1,NIU Shan-guang1,LIU Xing-hong1,LIU Yu-sheng2,JIN Ying3(1.Shangdong Provincial Academy of Forestry,Ji'nan 250014,2.College of Plant Protection,Shandong Agricultural University,Tai'an,271018,3.Huangdao Entry-Exit Inspection and Quarantine Bureau,Qingdao,266000) |
| Abstract: | Four antagonistic bacteria(numbered PF-1,PF-2,Bs-03,Bf-02),which were isolated from soil,were strongly inhibiting to the growth of the Colletotrichum gloeosporioides.In this paper,identification of four antagonistic bacteria and its inhibition efficacy were studied.By observation of the morphological character,cultivation properties,measurements of the biochemistrical features,four strains were identified as Bacillus lacivae,B.brevis,B.subtilis,B.firmus respectively.Through dual-culture,their inhibition eff... |
| Keywords: | Antagonistic bacteria identification bacillus inhibition efficacy |
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