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| 洋桔梗叶片培养不定芽发生和微繁研究 | |
| 引用本文： | 达克东,张松,臧运祥,张志国,吴禄平.洋桔梗叶片培养不定芽发生和微繁研究[J].山东农业大学学报(自然科学版),2003,34(4):494-498. |
| 作者姓名： | 达克东 张松 臧运祥 张志国 吴禄平 |
| 作者单位： | 1. 山东农业大学,园艺学院,山东,泰安,271018;沈阳农业大学园艺学院,辽宁,沈阳,110161 2. 山东农业大学,园艺学院,山东,泰安,271018 3. 山东农业大学资源与环境学院,山东,泰安,271018 4. 沈阳农业大学园艺学院,辽宁,沈阳,110161 |
| 摘 要： | 以洋桔梗叶片为材料，建立了洋桔梗叶片高效不定芽发生、植株再生体系。研究了不同激素及其组合对外植体不定芽发生、壮苗和生根的效应。结果显示，MS BA 1mg／L是叶片不定芽发生的适宜培养基；MS BA 0．Img／L NAA 0．05mg／L是试管嫩梢形成壮苗的适宜培养基；MS IAA 0．1mg／L培养基是壮苗生根的适宜培养基。草炭珍珠岩基质是生根苗移栽的适宜基质。 |
| 关 键 词： | 洋桔梗 叶片 激素 不定芽发生 植株再生体系 培养基 微繁研究 |
| 文章编号： | 1000-2324(2003)04-0494-05 |
| 修稿时间： | 2002年11月5日 |
STUDY ON ADVENTITIOUS SHOOT REGENERATION AND MICRO-PROPAGATION FROM LEAVES OF LISIANTHUS (EUSTOMA GRANDIFLORUM) |
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| DA Ke - dong,ZHANG Song,ZANG Yun - xiang,ZHANG Zhi - guo,WU Lu - ping.STUDY ON ADVENTITIOUS SHOOT REGENERATION AND MICRO-PROPAGATION FROM LEAVES OF LISIANTHUS (EUSTOMA GRANDIFLORUM)[J].Journal of Shandong Agricultural University,2003,34(4):494-498. | |
| Authors: | DA Ke - dong ZHANG Song ZANG Yun - xiang ZHANG Zhi - guo WU Lu - ping |
| Abstract: | High frequency adventitious shoot formation and plant - let regeneration system were established in leaf culture of lisianthus (Eustoma Grandiflorum) . Effect of plant growth regulators and its combination on adventitious shoot regeneration, plant - let growth and plant - let rooting were studied. The results showed that MS basal medium supplemented with BA 1mg/L was suitable for leaf adventitious shoot formation, MS + BA 0. 1mg/L + NAA 0.05mg/L was suitable for plant - let growth, and MS + IAA 0. 1mg/Lmedium was suitable for plant - let rooting. 1/2 turfysoil + 1/2 vermiculite substrate was suitable for plant - let conversion in greenhouse. |
| Keywords: | eustoma grandiflorum leaf culture adventitious shoot micro - propagation |
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