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葡萄三倍体育种初报
引用本文:潘春云,亓桂梅.葡萄三倍体育种初报[J].山东农业大学学报(自然科学版),1998,29(3):298-302.
作者姓名:潘春云  亓桂梅
作者单位:山东省酿酒葡萄科研所
摘    要:用二倍体无核葡萄作母本与四倍体有核葡萄作父本进行杂交,用常规育种法去雄授粉,授粉后35d至55d期间,采用胚挽救技术,将各杂交组合的幼胚接种在不同培养基上进行离体培养。在设置的7个不同处理的杂交组合中,经系列培养后获5个杂交组合的胚培养苗19株。经根尖染色体鉴定,有一杂交组合的两组试管苗为三倍体。经3年继代培养,目前已形成两个葡萄三倍体新品系。

关 键 词:常规育种  幼胚培养  根尖染色体  葡萄三倍体

PRIMARY REPORT ON GRAPE-TRIPLOID BREEDING
Pan Chunyun,Qi Guimei,Tang Xiaoning,Yang Liying.PRIMARY REPORT ON GRAPE-TRIPLOID BREEDING[J].Journal of Shandong Agricultural University,1998,29(3):298-302.
Authors:Pan Chunyun  Qi Guimei  Tang Xiaoning  Yang Liying
Abstract:Hybridization of grape breeding was conducted by crossing the diploid seedless grape as female parent with the tetraploid seedless grape as male parent.Based on the general breeding method of cutting the stamens with pollenization,the hybridized young embryo was inoculated on the different media using saving embryo techniques in the period of 35 to 55 days after pollenization.Seven treatments of crossing associations were set up and nineteen embryo breeding seedlings were obtained by raising five crossing associations systematically.According to the root tip chromosome appraisal,two groups of test tube seedings from one hybridized association were triploid.After three-year continuous breeding,the seedlings had developed into two new varities of grape triploid.
Keywords:general breeding  young embryo raising  root tip chromosome  grape triploid
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