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| 用SSR标记建立玉米黄早四DNA标准指纹图谱的方法研究 | |
| 引用本文： | 张建华,张金渝,杨晓洪,肖植文,孔令明,华秋瑾,米艳华.用SSR标记建立玉米黄早四DNA标准指纹图谱的方法研究[J].西南农业学报,2006,19(3):345-350. |
| 作者姓名： | 张建华 张金渝 杨晓洪 肖植文 孔令明 华秋瑾 米艳华 |
| 作者单位： | 1. 云南省农业科学院质量标准与测试技术研究所,云南,昆明,650205;农业部植物新品种DUS测试(昆明)分中心,云南,昆明,650205 2. 云南省农业科学院药用植物研究所,云南,昆明,650223 3. 云南省农业科学院科研处,云南,昆明,650223 4. 云南省农业科学院质量标准与测试技术研究所,云南,昆明,650205 |
| 基金项目： | 云南省自然科学基金项目“玉米DUS测试技术体系构建”(2002C0078M)资助 |
| 摘 要： | DNA标准指纹图谱是分子检测技术用于玉米新品种选育及其DUS辅助评价的重要基础。通过玉米对黄早四DNA的SSR标准指纹图谱构建方法的研究,筛选出20对可以用于玉米DNA标准图谱构建的核心引物,它们的平均PIC值为0.8554,检测出的等位基因数是6~15个,平均8.55个,Bnlg125和Bnlg2162检测到了玉米黄早四的DNA特有谱带;8%的聚丙烯酰胺电泳体系可以将亲缘关系很近的玉米品种区分开来。对玉米DNA标准指纹图谱的建立方法和DNA取样方法进行了深入探讨。 |
| 关 键 词： | 黄早四 DNA标准指纹图谱 DUS测试 SSR标记 |
| 文章编号： | 1001-4829（2006）03-0345-06 |
| 收稿时间： | 2006-01-05 |
| 修稿时间： | 2006-01-05 |
The establishment of DNA standard fingerprint in maize variety Huangzao 4 by SSR markers |
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| ZHANG Jian-hua,ZHANG Jin-yu,YANG Xiao-hong,XIAO Zhi-wen,KONG Ling-ming,HUA Qiu-jin,MI Yan-hua.The establishment of DNA standard fingerprint in maize variety Huangzao 4 by SSR markers[J].Southwest China Journal of Agricultural Sciences,2006,19(3):345-350. | |
| Authors: | ZHANG Jian-hua ZHANG Jin-yu YANG Xiao-hong XIAO Zhi-wen KONG Ling-ming HUA Qiu-jin MI Yan-hua |
| Institution: | 1. Institute of Agricultural Quality Standard and Testing for Technology of Yunnan Academy of Agricultural Sciences(YAAS |
| Abstract: | The standard DNA fingerprint was an important tool in maize breeding and DUS testing of new variety.The method of building DNA standard fingerprint by SSR marker in Huangzao 4,a famous maize inbred line in China was studied.And 20 pairs core primers for DNA standard fingerprint building were screened out with PIC(polymorphism information content) values of 0.6754-0.903 by which 6-15 alleles were detected.And the characteristical bands of Huangzao 4 were detected by Bnlg125 and Bnlg2162.The cluster analysis with UPGMA from Nei's(1978) divided Huangzao 4 into 3 groups and 3 sub-groups.In addition,8 % polyacrylamide gel electrophoresis systems could detect more bands than 3 % metaphor gel electrophoresis,therefore it could be used for distinguishing maize varieties among close relative groups.The method for building DNA standard fingerprint of maize and DNA sampling were also discussed in this paper. |
| Keywords: | Huangzao 4 DNA standard fingerprint DUS testing simple sequence repeats marker |
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