| 检测新城疫抗体的Dot-ELISA方法的建立及其与HI方法的比较研究 |
| |
| 引用本文： | 黄艳艳,胡北侠,张秀美,沈志勇,刘玉庆,黄庆华,李俊.检测新城疫抗体的Dot-ELISA方法的建立及其与HI方法的比较研究[J].西南农业学报,2007,20(5):1117-1119. |
| |
| 作者姓名： | 黄艳艳 胡北侠 张秀美 沈志勇 刘玉庆 黄庆华 李俊 |
| |
| 作者单位： | 1. 山东省农业科学院畜牧兽医研究所,山东,济南,250100
2. 山东六和集团有限公司,山东,青岛,266061 |
| |
| 基金项目： | 山东省科技攻关资助项目(022020103) |
| |
| 摘 要： | 建立了检测鸡群中新城疫病毒(New Castle disease virus,NDV)血清抗体的Dot-ELISA方法,对Dot-ELISA的最佳反应条件进行了研究,初步确定了该方法的感染保护临界值为7log2。通过对大量临床血清样本的检测,表明Dot-ELISA与血凝抑制试验(HI)相比,具有敏感、特异和稳定性好的优点。
|
| 关 键 词： | 新城疫 血清抗体 Dot-ELISA HI |
| 文章编号： | 1001-4829（2007）05-1117-03 |
| 修稿时间： | 2006-10-11 |
Establishment of a Dot-ELISA method for the detection of ND antibody and its comparation with HI |
| |
| HUANG Yan-yan,HU Bei-xia,ZHANG Xiu-mei,SHEN Zhi-yong,LIU Yu-qing,HUANG Qing-hua,LI Jun.Establishment of a Dot-ELISA method for the detection of ND antibody and its comparation with HI[J].Southwest China Journal of Agricultural Sciences,2007,20(5):1117-1119. |
| |
| Authors: | HUANG Yan-yan HU Bei-xia ZHANG Xiu-mei SHEN Zhi-yong LIU Yu-qing HUANG Qing-hua LI Jun |
| |
| Abstract: | A Dot-ELISA method had been developed for the detection of ND serum antibody in chickens.The optimum condition of Dot-ELISA was determined and the antibody positive standard was established as 7log2.Dot-ELISA and HI were both used to detect ND antibody in chicken serums and the result indicated that Dot-ELISA was stabler and more sensible. |
| |
| Keywords: | ND serum antibody Dot-ELISA HI |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
