| 土壤根肿病菌休眠孢子PCR快速检测方法的建立 |
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| 引用本文： | 尹全,宋君,刘勇,刘红雨,黄小琴.土壤根肿病菌休眠孢子PCR快速检测方法的建立[J].西南农业学报,2010,23(2). |
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| 作者姓名： | 尹全 宋君 刘勇 刘红雨 黄小琴 |
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| 作者单位： | 1. 四川省农业科学院分析测试中心,四川,成都,610066
2. 四川省农业科学院植物保护研究所,四川,成都,610066 |
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| 基金项目： | 科技部国家科技支撑计划，四川省社会公益项目，四川省育种工程项目，四川省农科院博士后工作站项目资助 |
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| 摘 要： | 根据GenBank中根肿病菌的保守序列,设计了一对引物,该引物能从供试的根肿病菌根组织中特异性扩增出一条498bp的预期条带。采用改良土壤DNA提取方法对人工配制病土中根肿病菌休眠孢子DNA提取,通过检测不同稀释度和不同孢子量的DNA检测结果证明,该检测方法能检测出土壤根肿病菌含量大于104个孢子/g孢子浓度的带菌土壤,且该方法具有快速、准确、简便、有效的特点。
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| 关 键 词： | 根肿病菌 休眠孢子 土壤检测 |
Establishment of PCR Rapid Detection of Plasmodiophora brassica Resting Spores in Soils |
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| YIN Quan,SONG Jun,LIU Yong,LIU Hong-yu,HUANG Xiao-qin.Establishment of PCR Rapid Detection of Plasmodiophora brassica Resting Spores in Soils[J].Southwest China Journal of Agricultural Sciences,2010,23(2). |
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| Authors: | YIN Quan SONG Jun LIU Yong LIU Hong-yu HUANG Xiao-qin |
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| Institution: | YIN Quan1,SONG Jun1,LIU Yong2*,LIU Hong-yu2,HUANG Xiao-qin2 (1.Analysis , Testing Center,Sichuan Academy of Agricultural Sciences,Sichuan Chengdu 610066,China,2.Institute of Plant Protection,China) |
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| Abstract: | According to GenBank sequence of Plasmodiophora brassicae,a pair of primers were designed;the primers could specifically amplify an expected 498bp band from the infected root tissues.Through improving extraction method,disease soils prepared with resting spores by artificial method could be extracted for total DNA.In the case of different dilutions,the amount of different spores of total DNA test results showed that the detection method could detect that containing more than 104 spores / g in soils,and the ... |
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| Keywords: | PCR |
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