| 非洲菊叶外植体组培中影响褐化因素及机理初探 |
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| 引用本文： | 夏小环,王静,尹梅,陈志伟,陈善娜.非洲菊叶外植体组培中影响褐化因素及机理初探[J].西南农业学报,2006,19(1):136-138. |
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| 作者姓名： | 夏小环 王静 尹梅 陈志伟 陈善娜 |
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| 作者单位： | 1. 云南大学生命科学学院,云南,昆明,650091
2. 云南省农业科学院,云南,昆明,650205 |
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| 基金项目： | 云南省科技攻关项目（2001NT22） |
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| 摘 要： | 用5种不同成分的培养基分别在光照和黑暗条件下对非洲菊叶外植体进行组织培养,筛选出对褐化影响最重和最轻的分别是Fe盐加倍和1/2MS培养基。并对影响非洲菊叶片组培褐变中的几个相关酶活性和总酚含量变化做了一些初步研究,结果表明,随着褐化程度加深POD活性有下降趋势,PAL活性和总酚含量有上升趋势。为防止褐化,应在POD活性下降和PAL活性及总酚含量上升前加入抑制褐化的试剂。
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| 关 键 词： | 非洲菊 组织培养 褐化 POD PAL 总酚 |
| 文章编号： | 1001-4829（2006）01-0136-03 |
| 收稿时间： | 2005-09-21 |
| 修稿时间： | 2005-09-21 |
Study on factors and mechanism affecting browning of Gerbera jamesonii in tissue culture |
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| XIA Xiao-huan,WANG Jing,YIN Mei,CHEN Zhi-wei,CHEN Shan-na.Study on factors and mechanism affecting browning of Gerbera jamesonii in tissue culture[J].Southwest China Journal of Agricultural Sciences,2006,19(1):136-138. |
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| Authors: | XIA Xiao-huan WANG Jing YIN Mei CHEN Zhi-wei CHEN Shan-na |
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| Institution: | 1. College of Life Science, Yunnan University, Yunnan Kunming 650091, China; 2. Yunnan Academy of Agricultural Sciences, Yunnan Kunming 650205, China |
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| Abstract: | The leaf of flameray gerbera was cultured with five different culture media in dark and light respectively.Results showed that the worst and lightest browning were double Fe and 1/ 2MS culture medium separately.Studies on the changes of enzymic activity and phenol content concorned with browning indicated that the POD activity descended with the increasing of browning,while the PAL activity and phenol content increased with the degree of browning increasing.To prevent browning,reagents of anti-browning should be added before POD activity descends and PAL activity and phenol content increase. |
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| Keywords: | flameray gerbera tissue culture browning POD PAL phenol |
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