一组高温高效纤维素分解菌群的筛选及功能特性研究

从木质纤维材料堆肥样品中驯化筛选出一组高温型纤维素分解菌群N6,对其纤维素分解功能特性进行了研究.结果表明,N6培养体系的pH稳定在6.4～8.0之间,降解纤维的适宜温度为50～ 60℃,最高纤维素酶活(CMCase)为0.83 IU/mL；在50℃下,N6在4d内可使滤纸降解90.0％以上,6d内可使玉米秆粉或稻草粉分别降解75.0％和86.4％,9d内可使玉米秆粉或稻草粉分别降解失重79.0％和90.4％,对木薯渣的降解率也在30.0％以上.可见,N6具有降解天然木质纤维的高活性,是可应用于木质纤维材料生物转化的优良菌群.

Selection of A Theomorphic Microbial Community with High Efficiency in Cellulose Degradation and Its Function Character

A microbial community named as N6 was selected out from a lignocelluloses material compost sample,and its function on degrading cellulose was studied.The results showed that the N6 culture had a stable pH range of 6.4-8.0,own high activity in degrading cellulose at temperature of 50-60 ℃,with a highest cellulase(CMCase) activity of 0.83 IU/mL.Under 50 ℃,N6 could degrade above 90 % of filter paper within 4 d,degrade 75.0 % of corn straw powder and 86.4 % of rice straw powder within 6 d,79.0 % of corn straw powder and 90.4 % of rice straw power within 9 d,and also above 30 % of cassava residual.According to its high activity in degrading natural lignocelluloses,N6 would be a very good microbial community to be practically applied on the bio-converse of lignocelluloses materials.