| 甘蓝型油菜反义 Fad2 基因 RNAi 载体无选择标记转化研究 |
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| 引用本文： | 李兰玉,陈苇,杨清辉,罗延青,符明联,董云松,李根泽,王敬乔.甘蓝型油菜反义 Fad2 基因 RNAi 载体无选择标记转化研究[J].西南农业学报,2008,21(6). |
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| 作者姓名： | 李兰玉 陈苇 杨清辉 罗延青 符明联 董云松 李根泽 王敬乔 |
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| 作者单位： | 1. 云南农业大学农学与生物技术学院,云南,昆明,650201
2. 云南省农业科学院经济作物研究所,云南省农业生物技术重点实验室,云南,昆明,650223 |
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| 基金项目： | 云南省科技攻关计划，人才培养项目，国家自然科学基金 |
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| 摘 要： | 利用甘蓝型油菜种子特异性贮存蛋白基因cruciferin启动子及NOS终止子构建了无选择标记基因且含有反义结构的油酸脱饱和酶基因(Fad2)的RNA i表达载体。通过农杆菌介导法,获得了无选择标记转基因植株;再生植株的PCR检测表明,外源基因已高频率地整合到油菜基因组中,而且获得的转基因种子通过脂肪酸含量分析表明,油酸含量比对照有了很大的提高。
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| 关 键 词： | 甘蓝型油菜 Fad2基因 无选择标记 RNA干扰 基因转化 |
Research on marker-free transformation of Brassica napus with Fad2 antisense gene and RNA interference |
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| LI Lan-yu,CHEN Wei,YANG Qing-hui,LUO Yan-qing,FU Ming-lian,DONG Yun-song,LI Gen-ze,WANG Jing-qiao.Research on marker-free transformation of Brassica napus with Fad2 antisense gene and RNA interference[J].Southwest China Journal of Agricultural Sciences,2008,21(6). |
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| Authors: | LI Lan-yu CHEN Wei YANG Qing-hui LUO Yan-qing FU Ming-lian DONG Yun-song LI Gen-ze WANG Jing-qiao |
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| Abstract: | A RNA interference expression cassette for Fad2 gene,which is marker-free gene and with antisense structure,was constructed using the promotor of cruciferin gene from Brassica napus and NOS terminator.Transgenic rape plants without selection marker genes were obtained by Agrobacterium-mediated transformation.The results of PCR analysis indicated that Fad2 gene had been integrated into the genome of rapeseed in high efficiency.The fatty acid content analysis showed that the oleic acid content of transgenic seed was greatly increased as compared with control. |
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| Keywords: | Brassica napus Fad2 gene marker-free RNAi gene transformation |
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