油菜(B.juncea L.)温敏核不育的温度敏感基因分析

温敏核不育是雄性育性可受温度调节的一种不育类型.1999年云南省农科院从油菜(Brassica juncea L.)核不育材料"05S"中同时选育得到一个温敏核不育系"K121S"和一个不具温敏性的核不育两型系"116A".遗传试验表明,它们的雄性不育基因等位,不育性由核内2对隐性重叠基因控制,对温度敏感与否与另一对同不育基因互作的抑制基因有关,携带隐性纯合抑制基因的不育系(K121S),低温条件下其不育基因的作用被抑制而表现可育,高温条件下抑制基因作用失活,不育系仍表现雄性不育;携带显性纯合抑制基因的不育系(116A)其育性则不受温度影响.因此,在油菜温敏核不育"K121S"中,隐性抑制基因作用的温敏性导致了不育性的温敏性.

Analysis to the temperature sensitive gene in TGMS mustard (B.Juncea L.)

Thermo-sensitive genic male sterility (TGMS) is a type of male sterilities which male fertility can be influenced by temperature. A TGMS line "K121S" and a GMS line "116A" of without temperature sensitivity in rapeseed mustard (Brassicajuncea L.) was developed from a same recessive genic male sterile (GMS) material "05S" in 1999 in YAAS. "K121S" showed stable sterile under higher temperature (mean daily average＞18.2℃) but fertile under lower temperature (mean daily average ＜10.8℃) .Genetic experiments indicated there was 2 pairs of recessive duplicate genes controlled their male sterility. Fertility is sensitive to temperature or no was decided by a pair of suppressor gene which interacting with sterile gene. Sterile line carrying homozygous recessive inhibiting gene (K121S) , the role of sterile genes can be restrained under lower temperature and induced male fertile, but under higher temperature the inhibiting gene failed activity and sterile line will still express male sterile. The sterile line carrying homozygous dominant inhibitor gene (116A) , which fertility was not to be affected by temperature and always kept 1:1 ratio of fertile to sterile in sister-cross generation. So that, in the TGMS mustard "K121S" , the recessive suppressor gene was a real temperature sensitive gene.