| 香料烟粉虱传双生病毒的分子检测 |
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| 引用本文： | 董家红,李光西,罗延青,丁铭,张丽珍,张仲凯.香料烟粉虱传双生病毒的分子检测[J].西南农业学报,2007,20(3):417-420. |
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| 作者姓名： | 董家红 李光西 罗延青 丁铭 张丽珍 张仲凯 |
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| 作者单位： | 1. 云南省农业生物技术重点实验室,云南,昆明,650223
2. 保山香料烟有限责任公司,云南,保山,678000 |
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| 基金项目： | 国家自然科学基金(30360005)，云南省烟草专卖局研究项目(03A34) |
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| 摘 要： | 发生在保山香料烟生产区粉虱传双生病毒病,已严重影响到了香料烟的品质和产量。本研究利用PCR技术快速检测香料烟植株及田间其它植物如胜红蓟、赛葵及菜豆等、以及病毒介体的带毒情况。对部分样品的PCR检测及序列分析表明,感染香料烟双生病毒有中国番茄黄曲叶病毒、番茄黄曲叶病毒、云南烟草曲茎病毒、烟草曲茎病毒以及云南烟草曲叶病毒等,且一些样品存在复合侵染现象。本研究为掌握病害发生流行的基本规律,监控病害在香料烟上的发生流行提供了依据。
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| 关 键 词： | 粉虱传双生病毒 香料烟 PCR检测 |
| 文章编号： | 1001-4829（2007）03-0417-04 |
| 修稿时间： | 2006-05-05 |
Molecular detection of whiteflies-transmitted geminiviruses infecting uriental tobacco |
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| DONG Jia-hong,LI Guang-xi,LUO Yan-qing,DING Ming,ZHANG Li-zhen,ZHANG Zhong-kai.Molecular detection of whiteflies-transmitted geminiviruses infecting uriental tobacco[J].Southwest China Journal of Agricultural Sciences,2007,20(3):417-420. |
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| Authors: | DONG Jia-hong LI Guang-xi LUO Yan-qing DING Ming ZHANG Li-zhen ZHANG Zhong-kai |
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| Institution: | 1. Yunnan provincial Key Laboratory for Agro-Biotechnology, Yunnan Kunming 650223, China ;2. Baeshan Oriental Tobacco Co. Ltd. , Yunnan Baoshan 678000 ,China |
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| Abstract: | The occurrence of Whiteflies-Transmitted Geminiviruses(WTGs) disease in Baoshan's oriental tobacco planting areas has a severe effect on the quality and yield of oriental tobacco.This study detected rapidly WTGs infecting oriental tobacco,and weeds such as Ageratum,malvastrum and bean surrounding the oriental tobacco field,and vector-transmitted WTGs by PCR.PCR detection and sequence analysis of some samples revealed WTGs infecting oriental tobacco consist of Tomato yellow leaf curl China virus,Tomato yellow leaf curl virus,Tobacco curly shoot Yunnan virus,Tobacco curly shoot virus and Tobacco leaf curl Yunnan virus.Some samples were infected complexly by these viruses.This study provides the research foundation for holding the basic mechanism of the occurrence and prevalence of WTGs and to monitor the disease. |
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| Keywords: | whiteflies-transmitted geminiviruses oriental tobacco PCR detection |
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