| 小麦1BL/1RS易位系的生化和分子标记鉴定 |
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| 引用本文： | 吴崇明,徐智斌,王涛.小麦1BL/1RS易位系的生化和分子标记鉴定[J].西南农业学报,2007,20(1):15-18. |
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| 作者姓名： | 吴崇明 徐智斌 王涛 |
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| 作者单位： | 中国科学院成都生物研究所,四川,成都,610041 |
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| 基金项目： | 中国科学院知识创新工程重要方向、四川省科技厅项目资助 |
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| 摘 要： | 本文利用A-PAGE方法对87份供试的小麦材料进行了黑麦碱(secalin)检测,并进一步利用低分子量麦谷蛋白Glu-B3的STS-PCR标记以及与抗条锈病基因Yr9紧密连锁的Xgwm582标记对上述材料进行了1BL/1RS易位检测。结果表明,在87份材料中鉴定出了一种新的改良1BL/1RS易位系,该类易位系的特征是:含有来自1RS的抗条锈病基因Yr9、不含黑麦碱、具有Glu-B3基因、比一般1BL/1RS易位系具有明显偏高的SDS-沉降值。本研究中选用的生化标记和分子标记可以有效检测出育种材料中的1BL/1RS、非1BL/1RS以及改良1BL/1RS易位系。
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| 关 键 词： | 小麦 Glu-B3 Yr9 Sec-1b 1BL/1RS易位系 |
| 文章编号： | 1001-4829（2007）01-0015-04 |
| 修稿时间： | 2006-08-12 |
Biochemical and molecular markers for identification of 1BL/1RS translocation in Wheat |
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| WU Chong-ming,XU Zhi-bing,WANG Tao.Biochemical and molecular markers for identification of 1BL/1RS translocation in Wheat[J].Southwest China Journal of Agricultural Sciences,2007,20(1):15-18. |
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| Authors: | WU Chong-ming XU Zhi-bing WANG Tao |
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| Institution: | Biological Research Institute of Chengdu, Chinese Aeadc my of Science, Sichuan Chengdu 610041, China |
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| Abstract: | The presence of secalin was determined by A-PAGE and 1BL/1RS translocation was characterized by PCR markers in 87 wheat materials in this study.The markers applied were Glu-B3 STS-PCR marker and Xgwm582marker which tightly linked with stripe rust resistance gene Yr9.The results indicated that an improved 1BL/1RS translocation has Glu-B3 and Yr9 without secalin,and has higher SDS-sedmentation value than common 1BL/1RS translocation.The result also showed that the biochemical and molecular markers in this research could be applied to identify the presence of 1BL/1RS translocation as well as the type of such translocation. |
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| Keywords: | Wheat Glu-B3 Yr9 Sec-1b 1BL/1RS translocation |
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