| 小西瓜茎尖离体再生体系的建立 |
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| 引用本文： | 刘独臣,房超,刘小俊,梁根云,李跃建.小西瓜茎尖离体再生体系的建立[J].西南农业学报,2008,21(5). |
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| 作者姓名： | 刘独臣 房超 刘小俊 梁根云 李跃建 |
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| 作者单位： | 四川省农科院园艺研究所,四川成都,610066 |
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| 基金项目： | 四川省财政育种攻关资金项目 |
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| 摘 要： | 以茎尖为外植体,进行小西瓜离体快繁研究,建立了小西瓜茎尖再生体系。结果表明:小西瓜种子发芽的最适培养基为2FeMS;最适茎尖丛芽诱导培养基为MS1 BA 1~2 mg/L IAA 0.01 mg/L,增殖系数高;最适继代培养基为MS4 BA 0.2 mg/L NAA 0.05 mg/L;不定芽在培养基1/2MS 0.05 mg/L IAA上生根良好。
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| 关 键 词： | 小西瓜 组织培养 快繁 |
Establishment of the shoot-tip culture system in vitro in miniwatermelon |
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| LIU Du-chen,FANG Chao,LIU Xiao-jun,LIANG Gen-yun,LI Yue-jian.Establishment of the shoot-tip culture system in vitro in miniwatermelon[J].Southwest China Journal of Agricultural Sciences,2008,21(5). |
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| Authors: | LIU Du-chen FANG Chao LIU Xiao-jun LIANG Gen-yun LI Yue-jian |
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| Abstract: | The techniques of tissue culture and rapid propagation in vitro on miniwatermelon were studied by using shoot-tip an explants.A good culture system for miniwatermelon was established.The results showed the optimal germination medium was 2FeMS,the optimized induction medium for shoot-tip was MS1 BA 1-2 mg/L IAA 0.01 mg/L,in which the propagation ratio was high.the suitable medium of subculture was MS4 BA 0.2 mg/L NAA 0.05 mg/L,and tie rootage medium was 1/2MS 0.05 mg/L IAA. |
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| Keywords: | small-sized watermelon shoot-tip culture in vitro regrowth system |
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