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| 云南水稻条纹病毒RNA3的分子变异及遗传多样性分析 | |
| 引用本文： | 龙亚芹,王万东,李凡,杨文娟,陈海如.云南水稻条纹病毒RNA3的分子变异及遗传多样性分析[J].西南农业学报,2011,24(2). |
| 作者姓名： | 龙亚芹 王万东 李凡 杨文娟 陈海如 |
| 作者单位： | 1. 云南农业大学农业生物多样性与病虫害控制教育部重点实验室,云南昆明650201;云南省农业科学院热带亚热带经济作物研究所,云南保山678025 2. 云南省农业科学院热带亚热带经济作物研究所,云南保山,678025 3. 云南农业大学农业生物多样性与病虫害控制教育部重点实验室,云南昆明,650201 |
| 摘 要： | 为从分子水平上探讨云南水稻条纹病毒(RSV)分子变异及其遗传多样性,采用RT-PCR技术,对云南省大理州巍山县和昆明市宜良县2个分离物的RNA3进行克隆和测序,获得RNA3YWS和RNA3YYL全长序列,2个分离物的RNA3核苷酸序列长度分别为2489和2490bp.并将获得的序列与不同时期、不同区域的RNA3全长序列进行比较,并对22条RNA3序列构建系统发育树.结果表明,YWS和YYL与YBS、YA、FYi等分离物具有更近的亲缘关系,与云南以外的中国分离物和日本T和M分离物亲缘关系较远,表明RSV在自然界中的分子变异与地理分布有着密切的关系.根据不同基因在核苷酸和氨基酸水平上的差异,探讨了22条RNA3的分组情况,YWS和YYL同属于第一组,即云南组;同时对RNA32个编码基因及IR3在DNA水平上的遗传多样性进行分析,综合分析了云南RSV不同分离物RNA3的分子变异.推测云南独特的地理气候条件和丰富的生物多样性可能是造成云南RSV复杂遗传分化的主要原因. |
| 关 键 词： | 水稻条纹病毒 RNA3 分子变异 遗传多样性 |
Molecular Variation and Genetic Diversity Analysis of Rice Stripe Virus in RNA3 Complete Sequences in Yunnan |
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| LONG Ya-qin,WANG Wan-dong,LI Fan,YANG Wen-juan,CHEN Hai-ru.Molecular Variation and Genetic Diversity Analysis of Rice Stripe Virus in RNA3 Complete Sequences in Yunnan[J].Southwest China Journal of Agricultural Sciences,2011,24(2). | |
| Authors: | LONG Ya-qin WANG Wan-dong LI Fan YANG Wen-juan CHEN Hai-ru |
| Institution: | LONG Ya-qin1,2,WANG Wan-dong2,LI Fan1,YANG Wen-juan1,CHEN Hai-ru1(1.Key Laboratory for Agricultural Biodiversity and Pest Management of China Education Ministry,Yunnan Agricultural University,Yunnan Kunming 650201,China,2.Institute of Tropical and Subtropical Cash Crops,Yunnan Academy of Agricultural Sciences,Yunnan Baoshan 678025,China) |
| Abstract: | In order to explore the RSV molecular variation and genetic diversity at the molecular level,the complete sequences of RNA3YWS and RNA3YYL were cloned and sequenced by RT-PCR.The results showed that the length of RNA3 of YWS and YYL were 2489 and 2490 bp respectively.By comparing two sequences with the sequences of the RSV isolates reported previously in different periods,different areas,the nearer relationship among YWS,YYL and YBS,Y A,FYi and the farther relationship with Chinese isolates outside of Yunna... |
| Keywords: | Rice stripe virus(RSV) RNA3 Molecular variability Genetic diversity |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |