| 高效有机磷降解BR133和BR57的分离和鉴定 |
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| 引用本文： | 康纪婷,吴翔,甘炳成,张小平.高效有机磷降解BR133和BR57的分离和鉴定[J].西南农业学报,2011,24(2). |
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| 作者姓名： | 康纪婷 吴翔 甘炳成 张小平 |
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| 作者单位： | 1. 四川农业大学资源与环境学院,四川雅安,625014
2. 四川省农业科学院土肥所微生物实验室,四川成都,610000 |
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| 摘 要： | 对四川贝尔农药厂污水处理池采集的活性污泥样品,进行了解磷细菌的分离和筛选,根据解磷圈的大小获得了27株解磷细菌,又通过钼锑抗比色法复筛出2株降解有机磷农药草甘膦性能较好且稳定的菌株BR13和BR57,其降解率均达到50%以上.对这2株菌进行了生理生化试验和16S rDNA序列分析,结果表明BR13为假单胞菌,BR57为摩根氏菌.
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| 关 键 词： | 解磷细菌 筛选 16S rDNA |
Isolation and Identification of High Efficiency Methlparathion Degrading Bacterium BR13 and BR57 |
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| KANG Ji-ting,WU Xiang,GAN Bing-cheng,ZHANG Xiao-ping.Isolation and Identification of High Efficiency Methlparathion Degrading Bacterium BR13 and BR57[J].Southwest China Journal of Agricultural Sciences,2011,24(2). |
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| Authors: | KANG Ji-ting WU Xiang GAN Bing-cheng ZHANG Xiao-ping |
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| Institution: | KANG Ji-ting1,WU Xiang2,GAN Bing-cheng2,ZHANG Xiao-ping1(1.College of Resources & Environment,Sichuan Agricultural University,Sichuan Ya'an 625014,China,2.Soil and Fertilizer Institute,Sichuan Academy of Agricultural Sciences,Sichuan Chengdu 610066,China) |
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| Abstract: | The methlparathion-degrading bacteria from activated sludge sample of wastewater treating pond of Sichuan Bei'er pesticide plant were isolated and identified.According to the size of the circle,27 methlparathion-degrading bacteria were obtained,and by anti-Mo-Sb colorimetric,the high efficient methlparathion-degrading bacteria BR13 and BR57 were rescreened out,which had the degrading rate up to 50 %.Based on the results of physiological and biochemical tests and 16S rDNA sequence analysis,strain BR13 was id... |
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| Keywords: | Methlparathion-degrading bacteria Screening 16S rDNA sequence |
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