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紫甘薯京薯6号脱毒苗的诱导与快繁
引用本文:段玉云,王新良,金月嵘,侯思名,罗红梅,曾黎琼.紫甘薯京薯6号脱毒苗的诱导与快繁[J].西南农业学报,2011,24(2).
作者姓名:段玉云  王新良  金月嵘  侯思名  罗红梅  曾黎琼
作者单位:1. 云南省农业科学院,云南昆明650231;云南省农业生物技术重点实验室,云南昆明650223
2. 云南省农业科学院生物技术与种质资源研究所,云南昆明650223;大理学院生命科学与化学学院,云南大理671000
3. 云南省农业科学院生物技术与种质资源研究所,云南昆明650223;云南农业大学园林园艺学院,云南昆明650201
4. 云南省农业科学院生物技术与种质资源研究所,云南昆明650223;云南省农业生物技术重点实验室,云南昆明650223
基金项目:昆明绿色经济示范区项目
摘    要:针对紫甘薯京薯6号生产现状,应用植物茎尖分生组织脱毒培养技术,对紫甘薯京薯6号脱毒苗的生产进行研究.结果显示,茎尖分生组织在添加0.75mg/L6-BA+0.1 mg/L NAA从的MS培养基上可诱导得到无毒苗,出芽率达到100%,出芽平均时间为28d,脱毒率达到95%.以紫甘薯京薯6号脱毒苗茎节为材料,在MS培养基中培养20~25d可以获得无毒快繁苗,繁殖系数为5~6倍.

关 键 词:紫甘薯京薯6号  脱毒苗  组织培养  快繁

Induction and Rapid Propagation of Virus-free Seedlings of Purple Sweet Potatoes Jingchu-6
DUAN Yu-yun,WANG Xin-liang,JIN Yue-rong,HOU Si-ming,LUO Hong-mei,ZENG Li-qiong.Induction and Rapid Propagation of Virus-free Seedlings of Purple Sweet Potatoes Jingchu-6[J].Southwest China Journal of Agricultural Sciences,2011,24(2).
Authors:DUAN Yu-yun  WANG Xin-liang  JIN Yue-rong  HOU Si-ming  LUO Hong-mei  ZENG Li-qiong
Institution:DUAN Yu-yun1,5,WANG Xin-liang2,3,JIN Yue-rong2,4,HOU Si-ming2,LUO Hong-mei2,ZENG Li-qiong2,5(1.Yunnan Academy of Agricultural Sciences,Yunnan Kunming 650231,China,2.Biotechnology and Genetic Germplasm Institute,Yunnan Academy of Agricultural Sciences,Yunnan Kunming 650223,3.College of Life Science and Chemistry,Dali University,Yunnan Dali 671000,4.College of Landscape and Horticulture,Yunnan Agricultural University,Yunnan Kunming 650201,5.Key Laboratory of Agricultural Biotechnology of...
Abstract:Based on its production situation of purple sweet potatoes Jingchu-6,some stem tips were used to induce virus-free seedlings by tissue culture in MS culture medium containing different 6-BA/NAA ratios.The results showed that the induction rate of the virus-free seedlings from the meristems of purple sweet potatoes Jinchu-6 reached 100 percent in MS medium containing 0.75 mg/L 6-BA and 0.1 mg/L NAA with a mean seedling time of 28 days,and the average rate of virus elimination was up to 95 percent.The reprodu...
Keywords:Purple sweet potato Jinchu-6  Virus-free seedling  Tissue culture  Rapid propagation  
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