| 大岩桐离体培养技术研究 |
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| 引用本文： | 何家涛,王会.大岩桐离体培养技术研究[J].西南农业学报,2007,20(4):867-869. |
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| 作者姓名： | 何家涛 王会 |
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| 作者单位： | 襄樊职业技术学院生物工程系,湖北,襄樊,441021 |
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| 基金项目： | 名优花卉组培快繁技术研究襄科技[2001]6号 |
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| 摘 要： | 以大岩桐带芽嫩茎为外植体,探讨不同外源激素水平对其不定芽分化、增殖与不定根形成的影响。结果表明:MS 噻二唑苯基脲(TDZ)1.0~2.0 mg/L IBA 0.1~0.3 mg/L培养基不定芽分化、增殖效果好,分化率达83.3%以上,增殖系数达6.0以上;1/2MS IAA 0.4 mg/L或1/2MS NAA 0.4 mg/L或1/2MS IBA 0.8 mg/L的生根效果好,生根率可达95.6%以上;在泥炭∶珍珠岩=1∶2的基质中驯苗,成活率可达95.3%。
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| 关 键 词： | 大岩桐 离体培养 再生植株 |
| 文章编号： | 1001-4829（2007）04-0867-03 |
| 修稿时间： | 2006-05-21 |
Studies on in vitro culture of Sinningia hybrid |
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| HE Jia-tao,WANG Hui.Studies on in vitro culture of Sinningia hybrid[J].Southwest China Journal of Agricultural Sciences,2007,20(4):867-869. |
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| Authors: | HE Jia-tao WANG Hui |
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| Institution: | Department of Biological Engineering,Vocational and Technology College, Hubei Xiangfan 441021 ,China |
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| Abstract: | Sinningia hybrid stem were used as the explant,exploring the effects of external hormone on adventitous buds differentiation multiplication and adventitious roots forming.The results show: In the medium of MS TDZ 1.0-2.0 mg/L IBA 0.1-0.3 mg/L,the effect on differentiation and multiplicationh of sprouts is good;the differentiation rate reach above83.3 % and multiplication rate reach over 6.0;in 1/2MS IAA 0.4 mg/L or 1/2MS NAA 0.4 mg/L or 1/2MS IBA 0.8 mg/L,the effect on radication is good;the rooting rate above a level of 95.6 %;using stroma of perlitehumus soil is 1:2 raising plants.the survival rate can reach a level of 95.3 %. |
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| Keywords: | Sinningia hybrid in vitro culture plantlet regeneration |
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