| 云南双江蔗区甘蔗宿根矮化病的调查及病原检测 |
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| 引用本文： | 李文凤,罗志明,黄应昆,王晓燕,尹炯.云南双江蔗区甘蔗宿根矮化病的调查及病原检测[J].西南农业学报,2012,25(4):1309-1312. |
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| 作者姓名： | 李文凤 罗志明 黄应昆 王晓燕 尹炯 |
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| 作者单位： | 云南省农业科学院甘蔗研究所,云南省甘蔗遗传改良重点实验室,云南开远661600 |
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| 基金项目： | 现代农业产业技术体系建设专项资金(CARS-20-2-2);云南省现代农业产业技术体系建设专项;云南省“人才培养”项目(2008PY087);农业科技成果转化资金项目(2010GB2F300434) |
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| 摘 要： | 甘蔗宿根矮化病(Patoon Stunting Disease,RSD)是一种世界性的重要甘蔗病害,对甘蔗生产危害极大.摸清蔗区RSD的分布、发生危害情况,是科学推广温水脱毒健康种苗,有效防控甘蔗宿根矮化病的关键.对云南双江蔗区甘蔗宿根矮化病的发生和分布进行了调查和田间采样,采用PCR和I-ELISA法,对田间采集的60个样本进行RSD检测.结果表明,51个样本为阳性,阳性检出率85％,9个样本为阴性,确认双江蔗区存在RSD；通过系统分析,明确了不同品种、不同植期、不同蔗地RSD发生状况,为双江蔗区推广应用温水脱毒健康种苗、有效防控甘蔗宿根矮化病提供科学依据.
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| 关 键 词： | 云南双江 甘蔗宿根矮化病 发生 检测 |
Survey and Pathogen Detection of Sugarcane Ratoon Stunting Disease in Shuangjiang,Yunnan |
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| LI Wen-feng
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LUO Zhi-ming
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HUANG Ying-kun
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WANG Xiao-yan
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YIN Jiong.Survey and Pathogen Detection of Sugarcane Ratoon Stunting Disease in Shuangjiang,Yunnan[J].Southwest China Journal of Agricultural Sciences,2012,25(4):1309-1312. |
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| Authors: | LI Wen-feng LUO Zhi-ming HUANG Ying-kun WANG Xiao-yan YIN Jiong |
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| Institution: | (Sugarcane Research Institute,Yunnan Academy of Agricultural Sciences,Yunnan Key Laboratory of Sugarcane Genetic Improvement,Yunnan Kaiyuan 661600,China) |
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| Abstract: | Ratoon stunting disease which causes tremendous damage to sugar cane production is an important sugarcane disease around the world.The key to control epidemic of the RSD and promote plant of disease free seedlings treated with warm water is to investigate the occurrence and distribution of RSD.In this study,60 sugarcane samples were collected from fields in Shuangjiang of Yunnan and then tested by PCR and I-ELISA.The results showed that of the tested 60 samples,51 samples(85.00 %) were RSD positive,confirming the presence of RSD in Shuangjiang.Moreover systematic analysis of RSD occurrence in different cultivars of different seasons and regions provided important information for controlling effectively RSD and amplifying size of disease free seedings in Shuangjiang. |
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| Keywords: | Shuangjiang Yunnna Ratoon stunting disease Occurrence Detection |
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