| 甘蔗与河八王属间杂种的SSR标记鉴定 |
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| 引用本文： | 刘昔辉,方锋学,张荣华,宋焕忠,杨荣仲,高轶静,区惠平,雷敬超,罗霆,段维兴,张革民,李杨瑞.甘蔗与河八王属间杂种的SSR标记鉴定[J].西南农业学报,2012(1):38-43. |
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| 作者姓名： | 刘昔辉 方锋学 张荣华 宋焕忠 杨荣仲 高轶静 区惠平 雷敬超 罗霆 段维兴 张革民 李杨瑞 |
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| 作者单位： | 中国农业科学院甘蔗研究中心;国家糖料改良中心广西甘蔗品种改良分中心;广西农业科学院甘蔗研究所;广西甘蔗遗传改良重点实验室;广西农业科学院农业资源与环境研究所 |
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| 基金项目： | 国家自然科学基金项目(31101195,30971837,30860150);国家科技支撑计划项目(2007BAD30B00);科技部国际合作项目(2008DFA30600,2009DFA30820);农业部948项目(2009-Z8);现代农业产业技术体系项目(CARS-20-3);广西自然科学基金项目(0991009Z,2010GXNSFB013027);广西农科院科技发展基金项目(200906Z,200923,G2010005);广西甘蔗研究所基本科研业务费项目(G2009008,G2009010,G2010005) |
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| 摘 要： | 以甘蔗属优良甘蔗新品种(系)为母本,以收集于广西的河八王属材料为父本进行有性杂交,利用SSR标记对获得的杂种进行真实性鉴定。从70对甘蔗SSR引物中筛选出9对用于对5个甘蔗×河八王组合的57个杂交后代植株进行鉴定,结果表明,其中有14株具有双亲特征带,确定为真杂种。SSR标记可用于甘蔗河八王杂种真实性鉴定,研究结果为甘蔗野生种质河八王属植物的杂交利用及相关基础研究奠定了良好的基础。
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| 关 键 词： | 甘蔗 河八王 SSR 杂种鉴定 |
Identification of Progenies from Sugarcane×Narenga porphyrocoma(Hance) Bor. by SSR Marker |
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| LIU Xi-hui,FANG Feng-xue,ZHANG Rong-hua,SONG Huan-zhong,YANG Rong-zhong,GAO Yi-jing,OU Hui-ping,LEI Jing-chao,LUO Ting,DUAN Wei-xing,ZHANG Ge-min,LI Yang-rui.Identification of Progenies from Sugarcane×Narenga porphyrocoma(Hance) Bor. by SSR Marker[J].Southwest China Journal of Agricultural Sciences,2012(1):38-43. |
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| Authors: | LIU Xi-hui FANG Feng-xue ZHANG Rong-hua SONG Huan-zhong YANG Rong-zhong GAO Yi-jing OU Hui-ping LEI Jing-chao LUO Ting DUAN Wei-xing ZHANG Ge-min LI Yang-rui |
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| Institution: | 1,2,3,4,5 (1.Sugarcane Research Center,Chinese Academy of Agricultural Sciences;2.Guangxi Sugarcane Variety Branch,National Sugar Crops Improvement Center;3.Sugarcane Research Institute,Guangxi Academy of Agricultural Sciences;4.Guangxi Key Laboratory of Sugarcane Genetic Improvement;5.Agricultural Resource and Enviroment Research Institute,Guangxi Academy of Agricultural Sciences,Guangxi Nanning 530007,China) |
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| Abstract: | In a bid to explore the feasibility of distant hybridization between sugarcane and Narenga porphyrocoma(Hance) Bor, SSR markers were used to identify the progenies from five crosses between sugarcane × Narenga porphyrocoma(Hance) Bor.Nine pairs of primers selected from 70 pairs of SSR primers were screened out and applied for identification of 57 progenies of sugarcane × Narenga porphyrocoma(Hance) Bor.The results showed that fourteen progenies had the special bands of parents,and they were thereby recognized as the true hybrids.SSR marker technology was proved to be useful for identifying the progenies of sugarcane × Narenga porphyrocoma(Hance) Bor.It could be concluded that this experiment established a favorable foundation on the effective use of hybrids from sugarcane and Narenga porphyrocoma(Hance) Bor as well as the related basic research. |
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| Keywords: | Sugercane Narenga porphyrocoma(Hance) Bor SSR marker Hybrids identification |
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