| 桑树二倍体及人工诱导的同源四倍体遗传差异的SRAP分析 |
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| 引用本文： | 林强,祁广军,赵卫国,朱方容,邱长玉,陈小青,方荣俊.桑树二倍体及人工诱导的同源四倍体遗传差异的SRAP分析[J].西南农业学报,2011,24(3). |
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| 作者姓名： | 林强 祁广军 赵卫国 朱方容 邱长玉 陈小青 方荣俊 |
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| 作者单位： | 1. 广西壮族自治区蚕业科学研究院，广西南宁，530007
2. 中国农业科学院蚕业研究所，江苏镇江，212018 |
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| 基金项目： | 现代农业产业技术体系建设专项(蚕桑)项目; 公益性行业(农业)科研专项经费项目(nyhyzx07-020-02); 广西自然科学基金项目(桂科自0991177); 广西科学研究与技术开发计划项目(桂科攻0992012,桂科攻10100010-4) |
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| 摘 要： | 利用SRAP分子标记技术,对8份桑树二倍体品种和经秋水仙碱化学诱变得到的同源四倍体进行DNA遗传差异研究和聚类分析。结果表明:桑树不同材料二倍体及其同源四倍体间的SRAP多态性有明显差异。农桑8号、璜桑37号、湖桑197号、湖桑199号和国桑20号的二倍体及其同源四倍体间的扩增条带完全一致,无差异,而育71-1二倍体及其同源四倍体的SRAP多态性最高,达到1.41%,所有的二倍体及其同源四倍体材料均聚在一起,不同材料间亲缘关系近的聚为一大类。说明不同桑树材料经秋水仙素诱变后产生的遗传差异较大,桑树二倍体及其同源四倍体产生变异的原因可能是染色体上的等位基因积加效应造成DNA分子遗传结构的改变。
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| 关 键 词： | 桑树 二倍体 同源四倍体 遗传差异 SRAP |
SRAP Analysis of Genetic Variation between Mulberry Diploid and Their Artificial Mutagenic Homologous Tetraploid |
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| LIN Qiang,QI Guang-jun,ZHAO Wei-guo,ZHU Fang-rong,QIU Chang-yu,CHEN Xiao-qing,FANG Rong-jun.SRAP Analysis of Genetic Variation between Mulberry Diploid and Their Artificial Mutagenic Homologous Tetraploid[J].Southwest China Journal of Agricultural Sciences,2011,24(3). |
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| Authors: | LIN Qiang QI Guang-jun ZHAO Wei-guo ZHU Fang-rong QIU Chang-yu CHEN Xiao-qing FANG Rong-jun |
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| Institution: | LIN Qiang1,QI Guang-jun1,ZHAO Wei-guo2,ZHU Fang-rong1,QIU Chang-yu1,CHEN Xiao-qing1,FANG Rong-jun2(1.Guangxi Academy of Sericulture Sciences,Guangxi Nanning 530007,China,2.Sericulture Research Institute,Chinese Academy of Agricultural Sciences,Jiangsu Zhenjiang 212018,China) |
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| Abstract: | SRAP molecular marker was used to study the DNA genetic variation and make cluster analysis between eight mulberry diploid varieties and its homological tetraploid which were chemically induced by colchicines.The results showed that SRAP polymorphism was significant different between diploid and homological tetraploid of different mulberry materials.There was no difference among amplified bands of diploid and homological tetraploid of Nongsang 8,Huangsang 37,Husang 197,Husang 199 and Guosang 20,while the po... |
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| Keywords: | Mulberry Diploid Homological tetraploid Genetic variation SRAP |
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