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| 魔芋块茎脱毒高效快繁体系的构建 | |
| 引用本文： | 吴毅歆,隋启君,谢庆华,陈海如.魔芋块茎脱毒高效快繁体系的构建[J].西南农业学报,2006,19(4):722-727. |
| 作者姓名： | 吴毅歆 隋启君 谢庆华 陈海如 |
| 作者单位： | 1. 云南省农科院经济作物研究所,云南,昆明,650205 2. 云南师范大学生物资源技术研究所,云南,昆明,650031 3. 云南农业大学云南省植物病理重点实验室,云南,昆明,650201 |
| 摘 要： | 以花魔芋块茎的中部组织为外植体,在12种培养基上诱导愈伤组织,筛选出M11(MS 6-BA 1.5 mg/L NAA 0.15 mg/L)为诱导愈伤组织最适培养基,诱导率达100%;以带单芽的愈伤组织块为外植体,在5种培养基上进行芽的分化,M9(MS 6-BA 1.0mg/L NAA 0.1 mg/L)为芽分化最适培养基,分化率达100%;M11为有效芽苗率最高的培养基,有效芽苗率达31.1%;随着继代次数的增加,脱毒率明显的提高,脱菌、毒率分别达100%、92.9%。 |
| 关 键 词： | 魔芋 花魔芋 脱毒 |
| 文章编号： | 1001-4829（2006）04-0722-06 |
| 收稿时间： | 2005-12-15 |
| 修稿时间： | 2005-12-15 |
System establishment of plant virus-free regeneration from tuber explants of Amorphophallus konjac |
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| WU Yi-xin,SUI Qi-jun,XIE Qin-hua,CHEN Hai-ru.System establishment of plant virus-free regeneration from tuber explants of Amorphophallus konjac[J].Southwest China Journal of Agricultural Sciences,2006,19(4):722-727. | |
| Authors: | WU Yi-xin SUI Qi-jun XIE Qin-hua CHEN Hai-ru |
| Institution: | 1. Cash Crop Research Institute, Yunnan Academy of Agricultutal Sciences, Yunnan Kunming 650205, China; 2. Institute of Biotechnology, Yunnan Normal University, Yunnan Kunming 650031, China;3. Key Laboratory for Plant Pathology of Yunnan Province, Yunnan Kunming 650201, China |
| Abstract: | Small tubers were inoculated in M1-M12 12 kinds of media,M11(MS 6-BA 1.5 mg/L NAA 0.15 mg/L)medium was suitable for callus induction,the induction rate reached 100 %;Explants were buds.Buds were inoculated in M8-M12 5 kinds of media.M9(MS 6-BA 1.0 mg/L NAA 0.1 mg/L)medium was suitable for differentiation of buds,the differentiation rate reached 100 %;M11 medium was suitable for efficient seedling induction,the efficiency of seedling rate reached 31.3 %;Rate of virus-free became high with number of subculture,the disease-free and the virus-free rate reached 100 %,92.9 %. |
| Keywords: | Amorphophallus konjac Amorphophallus konjac K Koch virus-free |
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