| 猪流感病毒NP基因的克隆及杆状病毒转移载体的构建 |
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| 引用本文： | 赵朴,郑玉姝,贾贝贝,乔传玲,陈化兰,刘兴友,李海燕.猪流感病毒NP基因的克隆及杆状病毒转移载体的构建[J].西南农业学报,2009,22(5). |
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| 作者姓名： | 赵朴 郑玉姝 贾贝贝 乔传玲 陈化兰 刘兴友 李海燕 |
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| 作者单位： | 1. 河南科技学院动物科学学院,河南,新乡,453003
2. 中国农业科学院哈尔滨兽医研究所,兽医生物技术国家重点实验室/农业部动物流感重点开放实验室,黑龙江,哈尔滨,150001 |
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| 摘 要： | 本研究通过RT-PCR扩增了猪流感病毒A/ Swine/ Inner Mongolia/ 547/ 01 (H3N2)的NP基因,并将其克隆入pMD 18-T载体.重组pMD 18-T经Sal I 和 EcoR I酶切后得到的NP基因插入pMelBacB载体的Sal I/EcoRI位点.PCR和限制性内切酶分析鉴定表明成功构建了pMelBacB-NP转移载体.这为开发NP诊断抗原和亚单位疫苗奠定了基础.
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| 关 键 词： | 猪流感病毒 NP基因 克隆 转移载体 |
Cloning of NP Gene of Swine Influenza Virus and Construction of Baculovirus Transfer Vector |
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| ZHAO Pu,ZHENG Yu-shu,JIA Bei-bei,QIAO Chuan-ling,CHEN Hua-lan,LIU Xing-you,LI Hai-yan.Cloning of NP Gene of Swine Influenza Virus and Construction of Baculovirus Transfer Vector[J].Southwest China Journal of Agricultural Sciences,2009,22(5). |
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| Authors: | ZHAO Pu ZHENG Yu-shu JIA Bei-bei QIAO Chuan-ling CHEN Hua-lan LIU Xing-you LI Hai-yan |
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| Abstract: | In the study,NP gene of A/ Swine/ Inner Mongolia/ 547/ 01 (H3N2)was amplified by RT-PCR. The product of RT -PCR was cloned into pMD 18-T vector. NP gene was inserted into the Sal I/EcoR I sites of pMelBacB after the recombinant pMD18-T was cleavaged with restriction endonuclease Sal I and EcoR I. Identification by PCR and restriction-endonuclease analysis showed that the recombinant transfer vector pMelBacB-NP was constructed successfully.It was helpful for development of diagnostic antigen and subunit vaccine based on NP. |
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| Keywords: | Swine influenza virus NP gene Cloning Transfer vector |
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