| 贵州黑山羊和黔北麻羊MSTN基因DraI酶切多态性分析 |
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| 引用本文： | 冯会利,石照应,田兴贵,曲月秀,主性,刘若余.贵州黑山羊和黔北麻羊MSTN基因DraI酶切多态性分析[J].西南农业学报,2012,25(1):282-285. |
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| 作者姓名： | 冯会利 石照应 田兴贵 曲月秀 主性 刘若余 |
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| 作者单位： | 贵州大学动物科学学院,贵州贵阳,550025 |
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| 基金项目： | 贵州省科技厅农业攻关项目“优质高产小香羊选育和养殖配套技术示范”[黔科合NY字(2008)3046];贵州省科学基金项目“贵州山羊肌肉生长抑制素基因多态性与生产性能相关性研究”[黔科合J字(2009)2136] |
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| 摘 要： | 以贵州黑山羊和黔北麻羊为材料,根据山羊MSTN基因序列(GenBank登录号:EF588035)设计1对引物,用PCR-RFLP法对该序列进行多态性分析。结果表明:PCR扩增片段207 bp处存在TTTTA的插入,导致出现1个DraI酶切多态性位点,黑山羊纯合野生型(AA)为优势基因型,杂合型(AB)和纯合突变型(BB)为非优势基因型,A等位基因为优势基因;黔北麻羊纯合野生型(CC)为优势基因型,杂合型(CD)和纯合突变型(DD)为非优势基因型,C等位基因为优势基因。
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| 关 键 词： | 贵州黑山羊 黔北麻羊 MSTN基因 PCR-RFLP 多态性 |
Polymorphism Analysis of MSTN Gene Digested with DraI of Guizhou Black Goat and Qianbei Ma Goat |
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| FENG Hui-li
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SHI Zhao-ying
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TIAN Xing-gui
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QU Yue-xiu
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ZHU Xing
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LIU Ruo-yu.Polymorphism Analysis of MSTN Gene Digested with DraI of Guizhou Black Goat and Qianbei Ma Goat[J].Southwest China Journal of Agricultural Sciences,2012,25(1):282-285. |
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| Authors: | FENG Hui-li SHI Zhao-ying TIAN Xing-gui QU Yue-xiu ZHU Xing LIU Ruo-yu |
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| Institution: | (College of Animal Science,Guizhou University,Guizhou Guiyang 550025,China) |
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| Abstract: | Black Goat and Qianbei Ma Goat were taken as materials in this study,a primer was designed based on goats MSTN genetic sequences(GenBank registration number: EF588035),and PCR-RFLP method was used to analyze the sequences polymorphism.The results showed that the TTTTA insert existed in 207 bp,led to the emergence of a DraI enzyme polymorphism site.Homozygous wild type of black goat was advantage genotype while heterozygous type(AB)and homozygous mutant type(BB) were disadvantage genotypes.A gene was a protogene;Meanwhile homozygous wild type of Qianbei Ma Goat(CC) was advantage genotype while heterozygous type(CD)and homozygous mutant type(DD) were disadvantage genotypes and C gene was a protogene. |
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| Keywords: | Goat MSTN gene PCR-RFLP Polymorphism |
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