仔猪多发性关节炎病原菌的分离鉴定及耐药性研究

从山东地区32个猪场发生多发性关节炎的仔猪体内分离到15株病原菌。通过对分离菌株的形态染色、分离培养、生化试验及种特异性基因的PCR扩增等方法鉴定菌株,结果显示4株分离菌株为副猪嗜血杆菌,7株为猪链球菌,2株为化脓隐秘杆菌,1株为猪胸膜肺炎放线杆菌,1株为猪丹毒杆菌。15株分离菌均对昆明小鼠有较强的致病力,且对先锋霉素类抗生素高度敏感,大多数菌株还对青霉素类、头孢菌素Ⅲ类、氟喹诺酮类、万古霉素、壮观霉素等抗生素高度敏感。该试验表明山东地区猪场仔猪多发性关节炎的病原菌主要是副猪嗜血杆菌和猪链球菌,可交替使用上述敏感抗生素防治该病。

Isolation, Identification and Antimicrobial Susceptibilities of Pathogens of Piglet Polyarthritis

A total of 15 pathogens of piglet polyarthritis were isolated from sick piglets in 32 pig farms in Shandong province.These bacterial isolates were identified by morphological staining,isolation and culture,biochemical tests and PCR amplification of species specific genes.Four isolates were identified as Haemophilus parasuis,seven isolates were identified as Streptococcus suis,two isolates were identified as Arcanobacterium pyogenes,one isolate was identified as Actinobacillus pleuropneumoniae,another isolate was identified as Erysipelothrix rhusiopathiae.The mice virulence test indicated these isolates were lethal to mice.Drug susceptibility tests showed that these isolates were all highly sensitive to cepovenin and most isolates were highly sensitive to penicillin antibiotics,cefaloglycin,fluoroquinolones,vancomycin and spectinomycin.It suggested that the pathogens of piglet polyarthritis in pig farms in Shandong province were mainly H.parasuis and S.suis and the sensitive antibiotics can be alternately administered to prevent and cure the disease.