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用微核技术检测农药对不同蚕豆的诱变效应
引用本文:孙雁霞,王跃华,苟兴华,苟小军,邬晓勇,李文娴.用微核技术检测农药对不同蚕豆的诱变效应[J].西南农业学报,2011,24(4).
作者姓名:孙雁霞  王跃华  苟兴华  苟小军  邬晓勇  李文娴
作者单位:成都大学生物产业学院,四川成都,610106
摘    要:根据发芽率、微核率参数从3种蚕豆种子中筛选最佳的微核实验材料,采用微核技术研究4种农药对蚕豆根尖细胞的诱变效应.结果表明:广元蚕豆种子是最佳的微核实验材料.4种农药单独使用时,农药浓度与微核率呈剂量-效应关系,敌敌畏的诱变效应最大,除它杀虫剂的诱变效应最小.4种农药的最佳使用浓度为:敌敌畏1.9 mg/L,敌杀死-溴氰菊酯2.5mg/L,甲氨基阿维菌素苯甲酸盐1.4 mg/L,除它强力广谱高效杀虫剂2.3 mg/L.

关 键 词:农药  蚕豆根尖  微核技术  诱变效应

Mutagenic Effects of Pesticides Detected by Micronucleus on Different Vicia faba Linn
SUN Yan-xia,WANG Yue-hua,GOU Xing-hua,GOU Xiao-jun,WU Xiao-yong,LI Wen-xian.Mutagenic Effects of Pesticides Detected by Micronucleus on Different Vicia faba Linn[J].Southwest China Journal of Agricultural Sciences,2011,24(4).
Authors:SUN Yan-xia  WANG Yue-hua  GOU Xing-hua  GOU Xiao-jun  WU Xiao-yong    LI Wen-xian
Institution:SUN Yan-xia,WANG Yue-hua,GOU Xing-hua,GOU Xiao-jun,WU Xiao-yong,LI Wen-xian (Department of Biological Industry,Chengdu University,Sichuan Chengdu 610106,China)
Abstract:To screen the best Vicia faba for the micronucleus experiment,three broad bean seeds were selected according to the germination rate and the micronucleus rate parameters.Four kinds of pesticides were used to inducce the Vicia faba root-tip by the technology of micronucleus.The results showed that: Guangyuan bean seed was the best Micronucleus test material.When each of the four pesticides was used alone,the pesticide concentration and micronuclei in a dose-response relationship.The maximum mutagenic effect ...
Keywords:Pesticides  Vicia faba root-tip  Micronucleus  Mutagenic effect  
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