应用ISSR分子标记研究茶树种质资源遗传多样性

采用ISSR分子标记技术对川渝茶树资源进行了遗传多态性分析。结果表明川渝茶树资源遗传多态性丰富,12条ISSR引物共产生100条扩增带,其中多态性条带90条(占90.0%)。ISSR标记遗传相似系数(GS)变异范围为0.41~0.87。通过类平均聚类(UPGMA)法,可将14份材料分为2个大类,第1大类主要为保持了较多原始性状的乔木大叶型,第2大类又可分成4个亚类群,主要为小乔木或灌木中小叶型,多为川渝各地栽培的地方群体品种。由此认为:ISSR标记可以有效地评价茶树种质的遗传多样性,并为其系统学研究提供分子依据。

Genetic diversity of tea camellias germplasm by ISSR molecular marker

ISSR molecular marker were used to analyze the genetic polymorphism of Sichuan-Chongqing tea camlias germplasm.The results showed that the genetic polymorphism of these materials were abundant.100 bands were amplified with 12 ISSR primers,and 90 bands of these were polymorphic ones(about 90 %).The genetic similarity of the materials varied between 0.41 and 0.87.The 14 materials were classified into 2 groups with UPGMA method,the first group is original arbor and large-leaf type,the second one is little-arbor or shrub type,and can be classified into 4 subgroups,mainly the cultivars of Sichuan-chongqing region.The results suggest that ISSR marker can be effectively used to evaluate the genetic polymorphism of tea camellias,and provide the foundation for the systematic research.