|
|||||
|
|
| 培养基成分对山参愈伤组织增殖生长的影响 | |
| 引用本文： | 修景润,廉美兰,朴成日,代月,朴炫春.培养基成分对山参愈伤组织增殖生长的影响[J].延边大学农学学报,2011,33(2):99-102. |
| 作者姓名： | 修景润 廉美兰 朴成日 代月 朴炫春 |
| 作者单位： | 1. 延边大学长白山生物功能因子省部共建教育部重点实验室,吉林延吉133002；延边朝鲜族自治州农业科学院,吉林龙井133400 2. 延边大学长白山生物功能因子省部共建教育部重点实验室,吉林延吉,133002 3. 延边朝鲜族自治州农业科学院,吉林龙井,133400 |
| 基金项目： | 延边大学“211工程”3期建设项目；中韩合作项目 |
| 摘 要： | 以从野山参根中诱导的愈伤组织为材料,研究MS培养基浓度、总氮和蔗糖浓度及水解酪蛋白浓度对愈伤组织增殖生长的影响.结果表明,愈伤组织在1.0和1.5 MS培养基中生长良好,当MS培养基中的总氮浓度为60 mM时,有利于野山参愈伤组织生长.MS基本培养基中加入蔗糖30 g/L和CH 1.0 g/L时,提高野山参愈伤组织鲜重、干重及增殖系数的增加. |
| 关 键 词： | 野山参 总氮 蔗糖 水解酪蛋白 |
Effect of medium component on callus growth of mountain ginseng in vitro |
|
| XIU Jing-run,LIAN Mei-lan,PIAO Cheng-ri,DAI Yue,PIAO Xuan-chun.Effect of medium component on callus growth of mountain ginseng in vitro[J].Journal of Agricultural Science Yanbian University,2011,33(2):99-102. | |
| Authors: | XIU Jing-run LIAN Mei-lan PIAO Cheng-ri DAI Yue PIAO Xuan-chun |
| Institution: | XIU Jing-run1,2,LIAN Mei-lan1,PIAO Cheng-ri2,DAI Yue1,PIAO Xuan-chun1(1.Key Laboratory of Nature Resource of Changbai Mountain & Functional Molecular(Yanbian University),Ministry of Education,Yanji,Jilin 133002,China,2.Yanbian Academy of Agricultural Sciences,Longjing Jilin 133400,China) |
| Abstract: | In order to promote callus proliferation and growth of mountain ginseng in vitro,the effects of MS strength,concentrations of nitrogen,sucrose,and hydrolyzed casein on callus growth were investigated.1.0 MS and 1.5 MS promoted callus growth;callus fresh weight,dry weight,and proliferation coefficient were favorable when MS medium supplemented with 60 mM nitrogen.30 g/L sucrose and 1.0 g/L hydrolyzed casein,the maximum proliferation coefficient reached 9.3. |
| Keywords: | mountain ginseng nitrogen sucrose hydrolyzed casein |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |