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| 封闭液对ELISA检测蓖麻毒素的影响 | |
| 引用本文： | 牛春华,刘文森,高宏伟,邢丽杰.封闭液对ELISA检测蓖麻毒素的影响[J].吉林农业科学,2008,33(5). |
| 作者姓名： | 牛春华 刘文森 高宏伟 邢丽杰 |
| 作者单位： | 1. 吉林省农业科学院,长春,130033 2. 军事医学科学院十一所,长春,130062 3. 军事医学科学院十一所,长春,130062;农业部食品质量监督检验测试中心,新疆,石河子,832000 |
| 摘 要： | 通过对30种封闭液与3种封闭条件对ELISA检测蓖麻毒素方法的封闭条件进行了优化,由P/N值得到1%酪蛋白+5%脱脂乳与5%脱脂乳+2%PEG 2万两种封闭剂在包被后孵育一次的封闭效果相似,也是所有组合中封闭效果最好的,从而大大提高了ELISA检测蓖麻毒素的敏感性和特异性。 |
| 关 键 词： | 封闭液 ELISA 蓖麻毒素 |
Effect of Block Agent on Detection of Ricin with ELISA |
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| NIU Chun-hua,LIU Wen-sen,GAO Hong-wei,XING Li-jie.Effect of Block Agent on Detection of Ricin with ELISA[J].Jilin Agricultural Sciences,2008,33(5). | |
| Authors: | NIU Chun-hua LIU Wen-sen GAO Hong-wei XING Li-jie |
| Abstract: | TO optimize the sensitivity and specificity of quantitative enzyme-linked immunosorbent assay(ELISA) for ricin,30 kids of block agent were selected.The P/N showed that 1% caseinum+5% skimmed milk,5% skimmed milk+2% PEG20000 were both effective.Simultaneously,3 kinds of blocking condition were compared.The P/N showed that adding block agent to coated 96 plate with anti-ricin,then incubated for 1 hour in 37℃,achieved the best blocking effect. |
| Keywords: | ELISA |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |