| 耐盐基因HAL1的克隆及在原核细胞中的表达 |
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| 引用本文： | 于志晶,韦正乙,卢东长城,蔡勤安,侯敬尧,邢少辰.耐盐基因HAL1的克隆及在原核细胞中的表达[J].吉林农业科学,2008,33(6). |
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| 作者姓名： | 于志晶 韦正乙 卢东长城 蔡勤安 侯敬尧 邢少辰 |
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| 作者单位： | 1. 吉林省农业科学院生物技术研究中心,长春,130033
2. 首都师范大学生命科学学院,北京,100000 |
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| 基金项目： | 吉林省科技厅科技发展计划
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| 摘 要： | 以酵母基因组DNA为模板,采用PCR方法得到耐盐基因HAL1,插入原核表达载体pGEX-4T-1的XhoI和EcoRI酶切位点之间,构建原核表达载体pGEX-HAL1;将该载体转化到大肠杆菌中,重组菌株用IPTG诱导表达,其耐盐性比空白菌株提高50%;SDS-PAGE凝胶电泳结果显示有明显表达的蛋白质条带。
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| 关 键 词： | 耐盐 克隆 大肠杆菌 载体 原核表达 |
Isolation of HAL1 Gene and Its Expression in Prokaryotic Cell |
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| YU Zhi-jing,WEI Zheng-yi,LUDONG Chang-cheng,CAI Qin-an,HOU Jing-yao,XING Shao-chen.Isolation of HAL1 Gene and Its Expression in Prokaryotic Cell[J].Jilin Agricultural Sciences,2008,33(6). |
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| Authors: | YU Zhi-jing WEI Zheng-yi LUDONG Chang-cheng CAI Qin-an HOU Jing-yao XING Shao-chen |
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| Institution: | YU Zhi-jing,WEI Zheng-yi,LUDONG Chang-cheng,CAI Qin-an,HOU Jing-yao,XING Shao-chen(Biotechnology Center,Academy of Agricultural Sciences of Jillin Province,Changchun 130033,China) |
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| Abstract: | With yeast genomic DNA as the template,HAL1 gene was amplified by PCR and ligated to the XhoI/EcoRI sites of pGEX-4T-1 vector,forming a new expression vector pGEX-HAL1.E.coli DH5α competent cells were transformed with this recombinant plasmid.The HAL1 protein was over ex-pressed in E.coli by IPTG induction.The resistance to NaCl of recombinant plasmid increased 50% com-pared with that of the control E.coli strain.About 59 kD expected protein band was separated using SDS-PAGE analysis. |
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| Keywords: | Salt tolerance Clone E coli Vector Prokaryotic expression |
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