| 玉米茎尖导入SAG_(12)基因的初步研究 |
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| 引用本文： | 曲文利,孙传波,孟凡梅,陶蕊,郭佳,李海华,张举仁,郝东云,袁英.玉米茎尖导入SAG_(12)基因的初步研究[J].吉林农业科学,2012,37(4):10-11,29. |
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| 作者姓名： | 曲文利 孙传波 孟凡梅 陶蕊 郭佳 李海华 张举仁 郝东云 袁英 |
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| 作者单位： | 1. 吉林省农业科学院生物技术研究中心,长春,130033
2. 山东大学生命科学学院,济南,250100 |
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| 摘 要： | 选用玉米优良自交系郑58的茎尖为受体,研究以玉米茎尖为受体进行农杆菌转化体系的可行性,用农杆菌介导法将耐盐碱基因SAG12转入玉米中,获得110株转化苗,经过300 mg/L的除草剂(Basta)筛选,共获得21株转基因植株。进一步进行PCR检测,其中14株表现阳性,转化率达12.73%。初步证明外源基因已经整合到玉米基因组中,以玉米茎尖作为受体孢子体的转化系统可用于基因转化。
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| 关 键 词： | 玉米 茎尖 农杆菌介导 SAG12基因 |
Transformation of SAG12 Gene into Apical Point of Maize Plant |
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| QU Wen-li
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SUN Chuan-bo
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MENG Fan-mei
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TAO Rui
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GUO Jia
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LI Hai-hua
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ZHANG Ju-ren
,
HAO Dong-yun
,
Yuan Ying.Transformation of SAG12 Gene into Apical Point of Maize Plant[J].Jilin Agricultural Sciences,2012,37(4):10-11,29. |
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| Authors: | QU Wen-li SUN Chuan-bo MENG Fan-mei TAO Rui GUO Jia LI Hai-hua ZHANG Ju-ren HAO Dong-yun Yuan Ying |
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| Institution: | 1*(1.Center of Agro-Biotechnology,Academy of Agricultural Sciences of Jilin Province,Changchun 130033; 2.College of Life Science,Shandong University,Jinan 250100,China) |
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| Abstract: | The SAG12 gene was transferred into the apical point of maize plant of inbred line ’Zheng 58’ by Agro-bacterium tumefaciens mediated transformation to study the feasibility of this system.21 transgenic plants were obtained after selecting with 300mg/L Basta and 14 of them were confirmed positive by PCR amplification.The frequency of transformation reached about 12.73%.The result showed that the foreign gene has integrated into maize genome and the apical point of maize plant can be used as receipt sporophyte for transformation. |
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| Keywords: | Maize Apical point Agro-bacterium tumefaciens mediated SAG12 |
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