| 吉林省粳稻盐胁迫响应差异表型和蛋白质分析 |
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| 引用本文： | 侯军梅,王丹,闫海生,马景勇.吉林省粳稻盐胁迫响应差异表型和蛋白质分析[J].吉林农业科学,2011,36(2). |
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| 作者姓名： | 侯军梅 王丹 闫海生 马景勇 |
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| 作者单位： | 吉林农业大学农学院,长春,130118 |
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| 基金项目： | 吉林省科技支撑项目(20070227); 长春市科技支撑项目(09KZ28); 吉林省财政厅作物育种项目(zx08001-027B) |
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| 摘 要： | 以吉林粳稻耐盐型吉8945和盐敏感型吉农大808为试验材料,通过双向聚丙烯酰胺凝胶电泳技术(2-DE)以及基质辅助激光解吸附离子化时间飞行质谱(MALDI-TOF-MS/MS)技术,分别对早期幼苗(10d苗龄)在正常和盐胁迫条件(100 mmol/L NaCl)下盐胁迫响应蛋白质组进行分析、鉴定,在叶片中,与对照相比,盐处理后吉8945有21个点明显上调,吉农大808中35个点上调表达,其中12个点在吉8945和吉农大808均上调表达且差异明显。选取其中6个点进行MALDI-TOF-MS/MS质谱分析,鉴定出两种蛋白质,分别为磷酸丙酮酸水合酶和核酮糖1,5-二磷酸羧化酶/加氧酶。
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| 关 键 词： | 水稻 盐胁迫 蛋白质 双向电泳 MALDI-TOF-MS |
Analysis on Differential Phenotype and Protein of Rice in Response to Salt Stress in Jilin Province |
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| HOU Jun-mei,WANG Dan,YAN Hai-sheng,MA Jing-yong.Analysis on Differential Phenotype and Protein of Rice in Response to Salt Stress in Jilin Province[J].Jilin Agricultural Sciences,2011,36(2). |
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| Authors: | HOU Jun-mei WANG Dan YAN Hai-sheng MA Jing-yong |
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| Institution: | HOU Jun-mei,WANG Dan,YAN Hai-sheng,MA Jing-yong(Jilin Agricultural University,Changchun 130118,China) |
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| Abstract: | Two hybrid rice strains,‘Ji 8945'(salt-tolerant) and‘Jinongda 808'(salt-sensitive),were used in this experiment.Two-dimensional polyacrylamide gel electrophoresis and MALDI-TOF-MS/MS techniques were employed to isolate and characterize salt stress responsive proteins of hybrid rice seedlings in early period of 10-day old under normal and salt stress(100mM NaCl) conditions.The results showed that 21 or 35 up-regulated spots were significantly found in‘Ji 8945'and‘Jinongda 808'leaves compared with the control.Six of them were selected and identified by MALDI-TOF-MS/MS.Only two proteins were identified,i.e.,ribulose-1,5-bisphosphate carboxylase/oxygenase and enolase. |
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| Keywords: | Rice Salt stress Protein Two-dimensional electrophoresis MALDI-TOF-MS |
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