新疆硝尔库勒盐湖放线菌分离方法的初步研究

为了研究新疆硝尔库勒盐湖中的放线菌多样性,以微生物抗高渗透压的生理机制为指导,结合盐湖高盐、寡营养的特点,设计了含有在嗜盐微生物细胞中起渗透调节作用的多种碳源和氮源的分离培养基,以TP培养基、GW1培养基和F6培养基为对照,对新疆硝尔库勒湖沉积物样品进行分离。结果表明,海藻糖-肌酸培养基(B3)分离效果最好,能分离到10个属16个不同种的放线菌;海藻糖-天冬氨酸培养基(B1)和海藻糖-谷氨酸培养基(B2)分离效果次之,均能分离到5个属7个不同种的放线菌,并有2个潜在的放线菌新物种被发现(菌株编号为41376、41293)。这说明以渗透调节物作为碳源和氮源是高盐环境放线菌分离比较理想的选择,为以后高盐环境微生物的分离培养提供了理论指导。

The Preliminary Investigation of Isolation Methods for Actinobacteria of Xiaoerkule Lake from Xinjiang

In order to study the diversity of actinobacteria in sediments of Xiaoerkule Saline Lake from Xinjiang,high osmotic pressure physiological mechanisms as a guide,with the Saline Lake high salt,oligotrophic characteristics,Culture media were optimized by provision of various combinations of different carbon sources and nitrogen sources which play an important role in osmoregulation of halophilic microorganisms.Compared with TP medium、GW1medium and F6 medium,the samples of Xiaoerkule Saline Lake from Xinjiang were separated.The results showed that trehalose-creatine medium(B3) is the best separation,and can be isolated from 10 genera and 16 species of actinomycetes;trehalose-aspartate medium(B1) and trehalose-glutamic acid medium(B2) followed by separation,although it can only be separated into five genera and 7 species of actinomycetes,but there are two potential new species of actinomycetes found(strain No.41376,41293).That the osmotic adjustment matter as a carbon source and nitrogen source is high salt environment actinomycetes isolated from the ideal choice for high salt environment,microbial isolation provided theoretical guidance.