金花葵多糖提取的工艺优化及抗氧化活性研究

【目的】优化金花葵多糖的提取工艺,考察金花葵多糖的抗氧化活性,为金花葵多糖的研究和利用提供参考依据。【方法】以金花葵多糖提取率为评价指标,在单因素试验基础上,采用L9(33)正交试验优化超声辅助提取工艺,通过对DPPH自由基和羟基自由基(·OH)清除能力的考察评价金花葵多糖的体外抗氧化活性。【结果】影响超声辅助提取金花葵多糖效果的因素排序为:超声时间料液比超声温度,其最佳提取工艺条件为:料液比1∶50、超声时间30min、超声温度50℃,在此条件下金花葵多糖的提取率为22.32%。自由基清除试验结果表明,金花葵多糖对DPPH自由基和·OH的清除率呈现剂量依赖性。【结论】优化得到的金花葵多糖提取工艺操作简单可行,提取的金花葵多糖具有较强抗氧化活性,该工艺可在金花葵多糖的提取研究和开发利用中应用。

Optimizing extraction technique of Hibiseu Smanihot L.polysaccharides and its antioxidant activity

Objective]The extraction process of polysaccharides from Hibiseu smanihot L.was optimized,and its antioxidant activity was investigated,in order to provide basis for research and utilization of polysaccharides from H.smanihot L.Method]Taking extraction rate of H.smanihot L.polysaccharides as index,and on the basis of single factor experiment,the extraction technology assisted by ultrasonic was optimized by L9(33) orthogonal test.The antioxidant activities of H.smanihot L.polysaccharides were evaluated through scavenging experiment on DPPH free radical and the hydroxyl free radical (· OH).Result]The three factors affecting ultrasonic-assisted extraction of H.Smanihot L.polysaccharides were in order as follows:ultrasonic time＞material-liquid ratio＞ultrasonic temperature.The optimum extraction process parameters of polysaccharides were as follows material-liquid ratio 1∶50,ultrasonic time of 30 min,ultrasonic temperature 50 ℃.Under such condition,the extraction rate reached 22.32％.Free radical scavenging experiment showed that scavenging effects of H.Smanihot L.polysaccharides towards DPPH free radical and · OH varied according to the dosage.Conclusion]The optimized extraction process of H.Smanihot L.polysaccharides is simple,convenient and feasible.And H.Smanihot L.polysaccharides has strong antioxidant activity.This processing technique can be applied in extraction and development of H.Smanihot L.polysaccharides.